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Journal ArticleDOI

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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Journal ArticleDOI
07 Sep 2010-PLOS ONE
TL;DR: Results indicate that taurine treatment improved As-induced hepatic damages by inhibiting PKCδ-JNK signalling pathways, and could provide a new approach for the reduction of hepatic complication due to arsenic poisoning.
Abstract: Background Oxidative stress-mediated hepatotoxic effect of arsenic (As) is mainly due to the depletion of glutathione (GSH) in liver. Taurine, on the other hand, enhances intracellular production of GSH. Little is known about the mechanism of the beneficial role of taurine in As-induced hepatic pathophysiology. Therefore, in the present study we investigated its beneficial role in As-induced hepatic cell death via mitochondria-mediated pathway.

94 citations


Cites methods from "A colorimetric method for the deter..."

  • ...ALT and ALP levels in the serum were measured by using standard kits according to the method of Rietman and Frankel [44] and Kind and King [45] respectively....

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Journal ArticleDOI
TL;DR: Several derivatives of isoniazid derivatives were synthesized in satisfactory yield and pharmacologically evaluated for their anti-inflammatory, analgesic, ulcerogenic, and lipid peroxidation activities by known experimental models.

93 citations

Journal ArticleDOI
TL;DR: Because of its appreciable efficacy in destroying intracellular parasites as well as non-hepatotoxic and non-nephrotoxic nature, harmine, in the vesicular forms, may be considered for clinical application in humans.
Abstract: Harmine, a beta-carboline amine alkaloid isolated from Peganum harmala, was tested for its antileishmanial properties both in vitro and in vivo. In vitro antileishmanial activity of harmine was encouraging and prompted us to confirm the activity in vivo in hamster models. Harmine was tested both in free form and in different vesicular forms viz. liposomes, niosomes and nanoparticles. The different vesicles were prepared by the published protocols. The percent intercalation of harmine in liposomes, niosomes and nanoparticles was found to be 65, 60 and 20, respectively, when determined at 325 nm (epsilon(M) =2.33 x 10 M(-1) cm(-1)). At an equivalent dose of 1.5 mg/kg body weight, injected subcutaneously (SC) for a total of six doses in 15 days, harmine was found to reduce spleen parasite load by approximately 40, 60, 70 and 80%, respectively in free, liposomal, niosomal and nanoparticular forms. An inverse relationship could be established between the efficacy in the lowering of spleen parasite load and the size of the vesicles. Specific biochemical tests related to normal liver and kidney functions revealed that the toxicity of the drug was reduced in the vesicular forms in the same order as their efficacy and the same was confirmed by the histopathological studies of splenic sections. Cell cycle analysis studies using flow cytometry suggested that although harmine interferes in the cell division stage, it does not induce apoptosis in Leishmania donovani promastigotes. The results using Confocal Microscopy supported that the cell death could be attributed to necrosis due to non-specific membrane damage. Even then, because of its appreciable efficacy in destroying intracellular parasites as well as non-hepatotoxic and non-nephrotoxic nature, harmine, in the vesicular forms, may be considered for clinical application in humans.

93 citations


Cites methods from "A colorimetric method for the deter..."

  • ...…the hamsters by cardiac puncture just before sacrifice and the sera were procured by centrifugation after about 3 h. Serum alkaline phosphatase was assayed according to the protocol of Bessey et al. (1946) and serum glutamate pyruvate transaminase (SGPT), according to Reitman and Frankel (1957)....

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Journal ArticleDOI
TL;DR: Results of laboratory screening of blood from 4800 patients attending a well population screening centre found significant findings with relation to changes in women during the menopausal period.

93 citations

Journal ArticleDOI
TL;DR: Zinc has the potential in alleviating the toxic effects of nickel in rat liver because of its property to induce metallothionein (S-rich protein) as a free radical scavenger, or its indirect action in reducing the levels of oxygen reactive species.

93 citations