A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It is suggested that protein catabolism is not increased with the onset of starvation, and there was increased excretion of potassium derived from an intracellular source in goldfish treated with cortisol.
93 citations
TL;DR: Treatment with either AgNP or Ag ions resulted in membrane damage and reduced the cell function of human liver cells, and oxidative stress markers indicated that oxidative stress might contribute to the toxicity effects of nano and ionic forms of silver.
Abstract: Scientific information on the potential harmful effects of silver nanoparticles (AgNPs) on human health severely lags behind their exponentially growing applications in consumer products. In assessing the toxic risk of AgNP usage, liver, as a detoxifying organ, is particularly important. The aim of this study was to explore the toxicity mechanisms of nano and ionic forms of silver on human hepatoblastoma (HepG2) cells. The results showed that silver ions and citrate-coated AgNPs reduced cell viability in a dose-dependent manner. The IC50 values of silver ions and citrate-coated AgNPs were 0.5 and 50 mg L(-1) , respectively. The LDH leakage and inhibition of albumin synthesis, along with decreased ALT activity, indicated that treatment with either AgNP or Ag ions resulted in membrane damage and reduced the cell function of human liver cells. Evaluation of oxidative stress markers demonstrating depletion of GSH, increased ROS production, and increased SOD activity, indicated that oxidative stress might contribute to the toxicity effects of nano and ionic forms of silver. The observed toxic effect of AgNP on HepG2 cells was substantially weaker than that caused by ionic silver, while the uptake of nano and ionic forms of silver by HepG2 cells was nearly the same. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 679-692, 2016.
93 citations
Cites methods from "A colorimetric method for the deter..."
...The activities of AST and ALT were measured spectrophotometrically using a-ketoglutarate reaction according to the standard method of Reitman and Frankel (1957)....
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TL;DR: It is shown that enhancing hepatic glycolysis reduces body weight and adiposity in obese mice and causes differential lipid profiles due to opposite effects on hepatic lipogenesis, associated with distinct phosphorylation states of carbohydrate response element binding protein and AMP-activated protein kinase.
93 citations
Cites methods from "A colorimetric method for the deter..."
...Plasma levels of alanine aminotranferease (ALT) and aspartate aminotransaminase (AST) were measured using colorimetric methods (Reitman and Frankel, 1957)....
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TL;DR: Treatment with diphenyl diselenide, at all doses, effectively protects the increase of lipid peroxidation when compared to 2-NP group.
92 citations