A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It is shown that such enzyme determinations undoubtedly justify a clinical interest and allow a description of certain factors involved in prognosis and give rise to hypotheses on the physiopathology of PMD.
Abstract: In 1949 Sibley and Lehninger, in a general study on serum aldolase, reported an increased activity in two patients with progressive muscular dystrophy (PMD). We thought then that an elevation in serum enzymes might represent an interesting basis for the diagnosis and, perhaps, for the search for a biochemical lesion of this disease, and we undertook a systematic survey. We studied in turn : aldolase (1953), phosphohexoisomerase (1954), transaminases (19.55), and lacticodehydrase (1957). We could show that such enzyme determinations undoubtedly justify a clinical interest and allow a description of certain factors involved in prognosis. They also give rise to hypotheses on the physiopathology of PMD.
92 citations
TL;DR: The results suggest that drugs that inhibit lipid peroxidation and oxidation of glutathione have no effect on conventional biochemical markers of liver injury and on liver histology of bile-duct-ligated rats for 7 days.
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TL;DR: It can be concluded that the lipophilic character of As antidotes may lead to unfavorable results following intraperitoneal administration.
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TL;DR: It can be concluded that toxic effects of chelating agents, including metal redistribution and hepatotoxicity, might be playing a crucial role in the metallothionein induction.
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TL;DR: The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway.
Abstract: The active constituent profile in Cape gooseberry (Physalis peruviana L.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway.
92 citations
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...Colorimetric determination of alanine aminotransferase or aspartate aminotransferase, ALT or AST, was estimated by measuring the amount of pyruvate or oxaloacetate produced by forming 2, 4dinitrophenylhydrazine according to the method of Reitman and Frankel [16]....
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...Colorimetric determination of alanine aminotransferase or aspartate aminotransferase, ALT or AST, was estimated by measuring the amount of pyruvate or oxaloacetate produced by forming 2, 4- dinitrophenylhydrazine according to the method of Reitman and Frankel [16]....
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