A combinatorial view of old and new RNA polymerase II modifications
Danielle E Lyons,Sarah McMahon,Melanie Ott +2 more
- Vol. 11, Iss: 2, pp 66-82
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TLDR
This work focuses on integrating newly identified and lesser-studied CTD post-translational modifications into the existing framework and reviews the growing body of work demonstrating crosstalk between different CTD modifications and the functional consequences of such crosStalk on the dynamics of transcriptional regulation.Abstract:
The production of mRNA is a dynamic process that is highly regulated by reversible post-translational modifications of the C-terminal domain (CTD) of RNA polymerase II. The CTD is a highly repetitive domain consisting mostly of the consensus heptad sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Phosphorylation of serine residues within this repeat sequence is well studied, but modifications of all residues have been described. Here, we focus on integrating newly identified and lesser-studied CTD post-translational modifications into the existing framework. We also review the growing body of work demonstrating crosstalk between different CTD modifications and the functional consequences of such crosstalk on the dynamics of transcriptional regulation.read more
Citations
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Functional organization of RNA polymerase II in nuclear subcompartments.
Karsten Rippe,Argyris Papantonis +1 more
TL;DR: In this paper , different ways of assembling the active RNA polymerase II transcriptional machinery relate to nuclear compartmentalization, and the concept of proteins and RNA phase-separating into liquid-like droplets was proposed to drive the formation of transcriptionally active subcompartments.
Journal ArticleDOI
What's all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry.
Blase M LeBlanc,R Yvette Moreno,Edwin E. Escobar,Mukesh Kumar Venkat Ramani,Jennifer S. Brodbelt,Yan Zhang +5 more
TL;DR: The development of various MS techniques are discussed and the pros and cons of each technique are highlighted to provide future investigators with a comprehensive overview of how MS can be used to investigate the complexities of RNAP-II mediated transcription.
Journal ArticleDOI
Control of non-productive RNA polymerase II transcription via its early termination in metazoans
TL;DR: In this article , the authors summarize current knowledge of how and when such promoter-proximal quality control is asserted on metazoan RNA polymerase II (Pol II) complexes.
Journal ArticleDOI
Control of non-productive RNA polymerase II transcription via its early termination in metazoans.
TL;DR: Current knowledge is summarized of how and when promoter-proximal quality control is asserted on metazoan Pol II, which is often followed by rapid degradation of the associated RNA.
Journal ArticleDOI
Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Dong-Hyuk Heo,Krzysztof Kuś,Pawel Grzechnik,Sue Mei Tan-Wong,Adrien Birot,Tea Kecman,Søren Nielsen,Nikolay Zenkin,Lidia Vasiljeva +8 more
TL;DR: In this paper, the histone deacetylase activity of the fission yeast Hos2/Set3 complex plays an important role in suppressing cryptic initiation of antisense transcription when RNA polymerase II phosphorylation is dysregulated due to the loss of Ssu72 phosphatase.
References
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