A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics
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Cites background from "A Common Mechanism of Cellular Deat..."
...In addition, recent evidence points towards a common mechanism of cell death involving disadvantageous cell responses to drug-induced stresses that are shared by all classes of bactericidal antibiotics, which ultimately contributes to killing by these drug...
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References
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"A Common Mechanism of Cellular Deat..." refers methods in this paper
...For additional pathway-level insights, we performed Gene Ontology-based enrichment (Ashburner et al., 2000; Camon et al., 2004) of the up- and downregulated gene lists using GO::TermFinder (Boyle et al., 2004), requiring pathway enrichment q values to be <0.05 and setting the p value estimation mode to bootstrapping....
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...Continued Gene Function ypeA putative acetyltransferase yraQ predicted permease yrhB hypothetical protein ytfA predicted transcriptional regulator a Pathway enrichment using Gene Ontology identified NADHcoupled electron transport (false discovery rate = 0.0345) as the only upregulated pathway common to all three bactericidal drug classes....
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...Interestingly, pathway enrichment (q value < 0.05) using Gene Ontology (Ashburner et al., 2000; Camon et al., 2004) found NADH-coupled electron transport (NADH dehydrogenase I) to be a key upregulated pathway common to all three bactericidal drug classes (Table 1)....
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...For additional pathway-level insights, we performed Gene Ontology-based enrichment (Ashburner et al., 2000; Camon et al., 2004) of the up- and downregulated gene lists using GO::TermFinder (Boyle et al., 2004), requiring pathway enrichment q values to be <0.05 and setting the p value estimation…...
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...For additional pathway-level insights, we performed Gene Ontology-based enrichment (Ashburner et al., 2000; Camon et al., 2004) of the up- and downregulated gene lists using GO::TermFinder (Boyle et al....
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14,389 citations
"A Common Mechanism of Cellular Deat..." refers methods in this paper
...Removal of the kanamycin-resistance cassette was accomplished using the pcp20 plasmid (Datsenko and Wanner, 2000) (Table S4) and confirmed by PCR prior to experimentation....
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...Positive P1 transductants were confirmed by acquisition of kanamycin resistance and PCR. Removal of the kanamycin-resistance cassette was accomplished using the pcp20 plasmid (Datsenko and Wanner, 2000) (Table S4) and confirmed by PCR prior to experimentation....
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9,239 citations
"A Common Mechanism of Cellular Deat..." refers methods in this paper
...For each time point in each bactericidal experiment set (norfloxacin, ampicillin, and kanamycin), we converted Dz scores to p values and chose significantly up- and downregulated genes by selecting those with a q value 0.05 (false discovery rate) (Storey and Tibshirani, 2003)....
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8,324 citations
"A Common Mechanism of Cellular Deat..." refers methods in this paper
...The resulting microarray *.CEL files were combined with *.CEL files from arrays that comprise the M3D compendium (Faith et al., 2007) (http://m3d.bu.edu; E_coli_v3_Build_3) and RMA normalized (Bolstad et al., 2003) with RMAExpress, for a total of 524 RMA-normalized E. coli expression arrays....
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...edu; E_coli_v3_Build_3) and RMA normalized (Bolstad et al., 2003) with RMAExpress, for a total of 524 RMA-normalized E....
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7,428 citations
"A Common Mechanism of Cellular Deat..." refers methods in this paper
...The recA, iscS, and TCA-cycle knockouts were constructed using P1 phage transduction and were derived from an E. coli single-gene knockout library (Baba et al., 2006) (Table S4)....
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