A confinable home and rescue gene drive for population modification
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TLDR
In this paper, a confinable population modification home-and-rescue (HomeR) drive was proposed for population modification in Drosophila targeting an essential gene.Abstract:
Homing-based gene drives, engineered using CRISPR/Cas9, have been proposed to spread desirable genes throughout populations. However, invasion of such drives can be hindered by the accumulation of resistant alleles. To limit this obstacle, we engineer a confinable population modification home-and-rescue (HomeR) drive in Drosophila targeting an essential gene. In our experiments, resistant alleles that disrupt the target gene function were recessive lethal and therefore disadvantaged. We demonstrate that HomeR can achieve an increase in frequency in population cage experiments, but that fitness costs due to the Cas9 insertion limit drive efficacy. Finally, we conduct mathematical modeling comparing HomeR to contemporary gene drive architectures for population modification over wide ranges of fitness costs, transmission rates, and release regimens. HomeR could potentially be adapted to other species, as a means for safe, confinable, modification of wild populations.read more
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Combating mosquito-borne diseases using genetic control technologies.
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Symbionts and gene drive: two strategies to combat vector-borne disease.
TL;DR: In this paper , Bhatt et al. reviewed the latest developments in both symbionts and gene drive-based methods, as well as distinctions and obstacles relating to these promising technologies.
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Toward a CRISPR-Cas9-based Gene Drive in the Diamondback Moth Plutella xylostella.
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Potential use of gene drive modified insects against disease vectors, agricultural pests and invasive species poses new challenges for risk assessment.
Yann Devos,John D. Mumford,Michael B. Bonsall,Ana M Camargo,Leslie George Firbank,Debora C.M. Glandorf,Fabien Nogué,Konstantinos Paraskevopoulos,Ernst A. Wimmer +8 more
TL;DR: In this article, the potential future development of new or additional risk assessment guidance for engineered gene drives (GDs) modified insects is discussed, as well as the areas where further risk assessment may be required.
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