Fig. 1 A conjugation platform for Cas9. a A modular design strategy to functionalize Cas9. b Structure-guided selection of chemical labeling sites. Protospacer adjacent motif-interacting (PI) domain is in blue, HNH domain is in yellow, RuvC domain is in cyan, recognition (REC) lobe is in magenta, and bridge helix (BH) is in grey. Crystal structure of the Cas9-gRNA-DNA ternary complex is used (PDB: 5F9R)22.
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