Fig. 3 ssODN display platform allows facile testing of multiple conditions. a HiBiT sequence knockin efficiency was increased at multiple genomic loci in U2OS cells (GAPDH and PPIB) or HEK-293FT cells (CFL1), (n= 3 biologically independent experiments). b The GFP11 sequence insertion at the GAPDH locus was promoted in HEK-293T cells (n= 3 biologically independent experiments). c HDR-mediated 12-base exchange efficiency at the CXCR4 locus was increased in HEK-293T cells (n= 3 biologically independent experiments). d Two-base exchange at the RBM20 locus was promoted in HEK-293FT cells. Unlabeled wild-type Cas9 (wt) and Cas9-adaptor conjugates labeled at the indicated residues were used (n= 3 biologically independent experiments). P-values were calculated by paired two-tailed t-test. Source data are provided as a Source Data file.
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