Fig. 5 Efficient engineering of INS-1E cells for secretion of exogenous peptides and proteins. a Schematic of genome editing in the Ins1 locus of INS-1E cells. PC, prohormone convertase. HDR, homology-directed repair. b Engineered cells can secrete exogenous gene products together with insulin. c INS-1E cells were engineered to secrete the 11-residue HiBiT peptide. Multiple gene insertion sites and DNA break sites were investigated (n= 2 biologically
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