A CRISPR/Cas9 system adapted for gene editing in marine algae
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261 citations
Cites background or methods from "A CRISPR/Cas9 system adapted for ge..."
...In this sense, CRISPR/Cas9 technology has been recently used to efficiently generate stable targeted gene mutations in microalgae, using P. tricornutum (Nymark et al., 2016) and Nannocloropsis as model organisms (Wang et al., 2016)....
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...tricornutum (Nymark et al., 2016) and Nannocloropsis as model organisms (Wang et al....
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Cites background from "A CRISPR/Cas9 system adapted for ge..."
...Now, it has been applied in other plants, including tomato, poplar, soya bean, petunia, marine algae and maize (Brooks et al., 2014; Char et al., 2016; Fan et al., 2015; Nymark et al., 2016; Sun et al., 2015; Zhang et al., 2016; Zhou et al., 2015)....
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168 citations
Cites background from "A CRISPR/Cas9 system adapted for ge..."
...Although technology for precision genome editing, including zinc-finger nucleases, transcription activator-like effector nucleases (TALENs), or CRISPR/Cas9, has been reported in many microalgae (Sizova et al., 2013; Weyman et al., 2015; Li et al., 2016; Nymark et al., 2016; Ajjawi et al., 2017), several challenges related to targeting, efficiency, and toxicity remain to be fully overcome....
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References
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