A critical assessment of the utility of protein-free splicing systems.
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"A critical assessment of the utilit..." refers background in this paper
...Group II self-splicing introns provide a natural precedent for RNA catalysis of the two chemical reactions of pre-mRNA splicing, and their well-established similarities and likely evolutionary relatedness to snRNAs (Pyle 2008), especially in light of the recent group II intron crystal structure (Toor et al. 2008), provide strong support for the hypothesis that the spliceosome active site is at least par-...
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...Although these core motifs, which are shared between U6 snRNA and group II introns, have recently been demonstrated to form the basis of the group II intron active site (Toor et al. 2008), similar sequences are also found in other ribozymes believed to be unrelated to snRNAs, and the full details of the role(s) of ACAGAGA and AGC in both these ribozymes and the spliceosome remain unclear....
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...…which are shared between U6 snRNA and group II introns, have recently been demonstrated to form the basis of the group II intron active site (Toor et al. 2008), similar sequences are also found in other ribozymes believed to be unrelated to snRNAs, and the full details of the role(s) of…...
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...…and their well-established similarities and likely evolutionary relatedness to snRNAs (Pyle 2008), especially in light of the recent group II intron crystal structure (Toor et al. 2008), provide strong support for the hypothesis that the spliceosome active site is at least partially RNA based....
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252 citations
"A critical assessment of the utilit..." refers background in this paper
...Relatedness of a ribozyme reaction to pre-mRNA splicing has conventionally been concluded from its sensitivity to mutation of the ACAGAGA and/or AGC motifs in the U6 snRNA (or its derivative) component of the reaction (Tuschl et al. 2001; Valadkhan and Manley 2001; Valadkhan et al. 2007)....
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...The ability of snRNA-derived RNA sequences to catalyze chemical reactions including 29–59 phosphodiester bond formation has previously been interpreted as strong evidence for ribozyme catalysis by the spliceosome (Tuschl et al. 2001; Valadkhan and Manley 2001; Valadkhan et al. 2007)....
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153 citations
"A critical assessment of the utilit..." refers background in this paper
...…and splicing in vitro: while many play important roles in the conformational rearrangements required for productive splicing (for review, see Smith et al. 2008), a growing body of evidence suggests that some spliceosomal proteins have a function beyond simple scaffolding of a catalytic RNA…...
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130 citations
"A critical assessment of the utilit..." refers background in this paper
...Magnesium has been directly demonstrated to be important for spliceosomal catalysis (Sontheimer et al. 1999; Gordon et al. 2000), and further investigation should clarify the coordination and precise mechanistic roles of divalent ions in the spliceosome....
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