A critical review on the environmental application of lipopeptide micelles.
TL;DR: In this article, a comprehensive summary of the recent knowledge advancement in genetically regulated lipopeptides production, micelles associated decontamination mechanisms in low temperature and high salinity environments, and up-to-date environmental applications is provided.
About: This article is published in Bioresource Technology.The article was published on 2021-07-17. It has received 19 citations till now.
Citations
More filters
01 Jun 2022
TL;DR: A fine understanding of Bacillus-forming Lipopeptides covering their characteristics, biosynthesis and regulation, mechanisms of action in antimicrobial activity, and utilizations and fabrications as a bio-preservative agent in the food industry is presented in this paper .
Abstract: Microbial infection is one of the biggest causes of food spoilage. Thus, preventing food from microbial-related spoilage has been one of the main concerns for the food community and the public. Exploring safe and efficient bio-preservatives has been on the way to the improvement of food quality. Lipopeptides (LPs), mainly secreted by Bacillus spp., are promising potential bio-preservatives, as they not only cover a broad range of antimicrobial functions against foodborne pathogens but inhibit food-deteriorating microorganisms as well. This review presents a fine understanding of Bacillus-forming LPs covering their characteristics, biosynthesis and regulation, mechanisms of action in antimicrobial activity, and utilizations and fabrications as a bio-preservative agent in the food industry. LPs show good potential in preserving food from deterioration, mainly in keeping the quality of vegetables, fruits, drinks, and aquatic products. The notable effects of LPs against microbial growth and food spoilage depend greatly on their types, structures and dosage. Moreover, the yield of LPs has been enhanced by gene regulation and the optimization of fermentation parameters. The nano-encapsulation of LPs as the core material is promising in guaranteeing their sustained release upon various triggers and promoting the preservation of food better. Therefore, there is a great need to explore the prospects of LPs formed by Bacillus spp. in the improvement of food preservation.
16 citations
TL;DR: In this paper , a structural causal model was built to estimate the strength of causal links among salinity, dispersant addition, cell abundance, biosurfactant productivity and oil biodegradation.
16 citations
TL;DR: In this paper, the authors summarized recent research progress in developing effective and environmentally friendly dispersants by using green, food grade, and biological surfactants and additives and highlighted the future research needs to promote the application of these new formulas in marine oil spill incidents.
Abstract: The dispersant application has been recognized as an effective marine oil spill response option. Sustainable dispersants are continuously formulated to minimize potential ecological concerns. This review summarized recent research progress in developing effective and environmentally friendly dispersants by using green, food grade, and biological surfactants and additives. The oil dispersion efficiency and toxicity of these newly generated dispersants have also been discussed. We ended this review by highlighting the future research needs to promote the application of these new formulas in marine oil spill incidents.
11 citations
TL;DR: In this article , the authors summarized recent research progress in developing effective and environmentally friendly dispersants by using green, food grade, and biological surfactants and additives and highlighted the future research needs to promote the application of these new formulas in marine oil spill incidents.
Abstract: The dispersant application has been recognized as an effective marine oil spill response option. Sustainable dispersants are continuously formulated to minimize potential ecological concerns. This review summarized recent research progress in developing effective and environmentally friendly dispersants by using green, food grade, and biological surfactants and additives. The oil dispersion efficiency and toxicity of these newly generated dispersants have also been discussed. We ended this review by highlighting the future research needs to promote the application of these new formulas in marine oil spill incidents.
11 citations
TL;DR: In this article , the authors reported the isolation and characterization of a biosurfactant producing bacterium Franconibacter sp. IITDAS19 from crude oil contaminated soil.
10 citations
References
More filters
TL;DR: This work reviews strategies for natural product screening that harness the recent technical advances that have reduced technical barriers and assess the use of genomic and metabolomic approaches to augment traditional methods of studying natural products.
Abstract: Natural products have been a rich source of compounds for drug discovery. However, their use has diminished in the past two decades, in part because of technical barriers to screening natural products in high-throughput assays against molecular targets. Here, we review strategies for natural product screening that harness the recent technical advances that have reduced these barriers. We also assess the use of genomic and metabolomic approaches to augment traditional methods of studying natural products, and highlight recent examples of natural products in antimicrobial drug discovery and as inhibitors of protein-protein interactions. The growing appreciation of functional assays and phenotypic screens may further contribute to a revival of interest in natural products for drug discovery.
1,822 citations
TL;DR: This work provides an update on the latest development of stabilized nZVI for various environmental cleanup uses, and overviews the evolution and environmental applications of stabilization, as well as revealing some critical knowledge gaps and research needs.
477 citations
TL;DR: The sequences for these molecules that can eliminate all curvature from the nanostructures they form in water and generate completely flat nanobelts with giant dimensions relative to previously reported systems are discovered.
Abstract: Many alkylated peptide amphiphiles have been reported to self-assemble into cylindrical nanofibers with diameters on the order of a few nanometers and micrometer scale lengths; these nanostructures can be highly bioactive and are of great interest in many biomedical applications. We have discovered the sequences for these molecules that can eliminate all curvature from the nanostructures they form in water and generate completely flat nanobelts with giant dimensions relative to previously reported systems. The nanobelts have fairly monodisperse widths on the order of 150 nm and lengths of up to 0.1 mm. The sequences have an alternating sequence with hydrophobic and hydrophilic side chains and variations in monomer concentration generate a "broom" morphology with twisted ribbons that reveals the mechanism through which giant nanobelts form. Interestingly, a variation in pH generates reversibly periodic 2 nm grooves on the surfaces of the nanobelts. With proper functionalization, these nanostructures offer a novel architecture to present epitopes to cells for therapeutic applications.
401 citations
TL;DR: It is found that pre-colonization of surfaces by S4Sm was critical for this bacterium to inhibit pathogen colonization and growth, and involves contributions from both biofilm formation and the production of the antibiotic TDA.
Abstract: Background: The probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this study, we investigated the specific roles of TDA secretion and biofilm formation in the probiotic activity of S4Sm. Results: Mutations in clpX (ATP-dependent ATPase) and exoP (an exopolysaccharide biosynthesis gene) were created by insertional mutagenesis using homologous recombination. Mutation of clpX resulted in the loss of TDA production, no decline in biofilm formation, and loss of the ability to inhibit the growth of Vibrio tubiashii and Vibrio anguillarum in co-colonization experiments. Mutation of exoP resulted in a ~60 % decline in biofilm formation, no decline in TDA production, and delayed inhibitory activity towards Vibrio pathogens in co-colonization experiments. Both clpX and exoP mutants exhibited reduced ability to protect oyster larvae from death when challenged by Vibrio tubiashii. Complementation of the clpX and exoP mutations restored the wild type phenotype. We also found that pre-colonization of surfaces by S4Sm was critical for this bacterium to inhibit pathogen colonization and growth. Conclusions: Our observations demonstrate that probiotic activity by P. inhibens S4Sm involves contributions from both biofilm formation and the production of the antibiotic TDA. Further, probiotic activity also requires colonization of surfaces by S4Sm prior to the introduction of the pathogen.
362 citations
TL;DR: Analysis of secondary metabolite biosynthesis clusters from diverse soil samples identifies a distinct class of calcium-dependent antibiotics—the malacidins—that bind lipid II and are active against multidrug-resistant Gram-positive pathogens.
Abstract: Despite the wide availability of antibiotics, infectious diseases remain a leading cause of death worldwide
1
. In the absence of new therapies, mortality rates due to untreatable infections are predicted to rise more than tenfold by 2050. Natural products (NPs) made by cultured bacteria have been a major source of clinically useful antibiotics. In spite of decades of productivity, the use of bacteria in the search for new antibiotics was largely abandoned due to high rediscovery rates2,3. As only a fraction of bacterial diversity is regularly cultivated in the laboratory and just a fraction of the chemistries encoded by cultured bacteria are detected in fermentation experiments, most bacterial NPs remain hidden in the global microbiome. In an effort to access these hidden NPs, we have developed a culture-independent NP discovery platform that involves sequencing, bioinformatic analysis and heterologous expression of biosynthetic gene clusters captured on DNA extracted from environmental samples. Here, we describe the application of this platform to the discovery of the malacidins, a distinctive class of antibiotics that are commonly encoded in soil microbiomes but have never been reported in culture-based NP discovery efforts. The malacidins are active against multidrug-resistant pathogens, sterilize methicillin-resistant Staphylococcus aureus skin infections in an animal wound model and did not select for resistance under our laboratory conditions. Analysis of secondary metabolite biosynthesis clusters from diverse soil samples identifies a distinct class of calcium-dependent antibiotics—the malacidins—that bind lipid II and are active against multidrug-resistant Gram-positive pathogens.
289 citations