Journal ArticleDOI
A dipyrrole derivative from Aloe vera inhibits an anti-diabetic drug target Dipeptidyl Peptidase (DPP)-IV in vitro.
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TLDR
The results of the studies suggested that the inhibition of the DPP-IV enzyme as one of the pathways by which the Aloe vera extract may restore the pancreatic islets cell mass in diabetic animal model.Abstract:
Aloe vera, a succulent herb, has a long history of use in traditional medicine, including diabetes. Earlier studies from our laboratory demonstrated that the Aloe vera extract has the ability to inhibit the diabetic drug target dipeptidyl peptidase (DPP) IV in vitro. This current study focuses on the isolation of small water soluble active molecule(s) involved in DPP-IV inhibition from Aloe vera extract, and further to characterize its structure and to elucidate the mode of inhibition of the DPP-IV enzyme. Aloe vera gel ethanolic extract was subjected to preparative reverse-phase high-pressure liquid chromatography (RP-HPLC), LH-20 Sephadex gel filtration chromatography, followed by analytical RP-HPLC, to isolate the active molecule involved in DPP-IV inhibition. Based on the spectroscopic studies, the structure of the isolated DPP-IV inhibitor was predicted to be 3, 6-dioxo-3, 3a, 6, 6 a-tetrahydropyrrolo [3, 4-c] pyrrole-1, 4-dicarboxamide with the chemical formula C8H6N4O4, having the molecular weight of 225.175 Da. This molecule inhibited the DPP-IV enzyme in a noncompetitive manner with an IC50 value of 8.59 ± 2.61 µM, with a Ki of 4.7 ± 0.038 µM. Thus, the mechanism of DPP-IV inhibition and the inhibitory constants were determined. The results of our studies suggested that the inhibition of the DPP-IV enzyme as one of the pathways by which the Aloe vera extract may restore the pancreatic islets cell mass in diabetic animal model.read more
Citations
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Journal ArticleDOI
Identification of Potential SARS-CoV-2 Main Protease and Spike Protein Inhibitors from the Genus Aloe : An In Silico Study for Drug Development.
Mohamed E. Abouelela,Hamdy K. Assaf,Reda A. Abdelhamid,Ehab S Elkhyat,Ahmed M. Sayed,Tomasz Oszako,Lassaad Belbahri,Ahmed E. El Zowalaty,Mohamed Salaheldin A. Abdelkader +8 more
TL;DR: In this article, the authors employed an in-silico approach involving molecular docking, dynamics simulation, and binding free energy calculation using SARS-CoV-2 essential proteins as main protease and spike protein to identify lead compounds from Aloe that may help in novel drug discovery.
Journal ArticleDOI
Dipeptidyl Peptidase (DPP)-IV Inhibitors with Antioxidant Potential Isolated from Natural Sources: A Novel Approach for the Management of Diabetes
TL;DR: In this article, a review of DPP-IV inhibitors and their mechanism of inhibition, activities of those isolated from various natural sources, and their capacity to overcome oxidative stress in disease conditions is presented.
Journal ArticleDOI
Alleviation of diabetes mellitus through the restoration of β-cell function and lipid metabolism by Aloe vera (L.) Burm. f. extract in obesogenic WNIN/GR-Ob rats.
Neha Deora,M.M. Sunitha,M. Satyavani,N. Harishankar,Mookambeswaran A. Vijayalakshmi,Krishnan Venkataraman,Vijayalakshmi Venkateshan +6 more
TL;DR: In this article, the authors investigated the simultaneous effect of ethanolic A. vera gel extract on diabetes and obesogenic milieu in Streptozotocin-induced WNIN/GR-Ob mutant obese rats.
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Antidiabetic potential evaluation of aqueous extract of waste Syzygium cumini seed kernel’s by in vitro α-amylase and α-glucosidase inhibition
TL;DR: The output of this research bestows support to utilize the SCKP stirred batch extract as a promising source of antioxidant and antidiabetic compounds in ayurvedic formulations.
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Downregulation of Candidate Gene Expression and Neuroprotection by Piperine in Streptozotocin-Induced Hyperglycemia and Memory Impairment in Rats.
Suresh Kumar,Suman Chowdhury,Ajay Razdan,Deepa Kumari,Ram Singh Purty,Heera Ram,Pramod Kumar,Prasunpriya Nayak,Sunil Shukla +8 more
TL;DR: In this paper, the authors evaluated the effect of piperine on Alzheimer's disease in diabetic rats and showed that piperines not only improved memory but also reduced the expression of specific AD-related genes.
References
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Journal ArticleDOI
Plasma dipeptidyl peptidase-IV activity in patients with type-2 diabetes mellitus correlates positively with HbAlc levels, but is not acutely affected by food intake
Jakob Ryskjær,Carolyn F. Deacon,Richard D. Carr,Thure Krarup,Sten Madsbad,Jens J. Holst,Tina Vilsbøll +6 more
TL;DR: Investigation of plasma DPP-IV activity in the fasting and the postprandial state in type-2 diabetic patients and control subjects found it was positively correlated to FPG and HbAlc levels and negatively to the duration of diabetes and age of the patients.
Journal ArticleDOI
Screening methods used to determine the anti-microbial properties of Aloe vera inner gel.
Fatema Habeeb,Eisin Shakir,Fiona Bradbury,Pamela Cameron,Mohamad R. Taravati,Allan J. Drummond,Alexander I. Gray,Valerie A. Ferro +7 more
TL;DR: Standard methods recommended by the Clinical and Laboratory Standards Institute are compared with a microtitre assay using a metabolic colour indicator Alamar blue, which enables high throughput screening, under similar conditions and is less wasteful of plant material.
Journal ArticleDOI
The Review on Properties of Aloe Vera in Healing of Cutaneous Wounds.
TL;DR: It is concluded that although aloe vera improves the wound healing as well as other procedures both clinically and experimentally, more studies are still needed to approve the outcomes.
Journal ArticleDOI
Berry and Citrus Phenolic Compounds Inhibit Dipeptidyl Peptidase IV: Implications in Diabetes Management.
TL;DR: Results indicate that flavonoids, particularly luteolin, apigenin, and flavone, and the stilbenoid resveratrol can act as naturally occurring DPP-IV inhibitors.
Journal ArticleDOI
Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates.
TL;DR: Nine novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides were identified in camel milk proteins hydrolysed with trypsin which may play a role in the regulation of glycaemia in humans.