Abstract: Pedestrian gender classification is one of the key assignments of pedestrian study, and it finds practical applications in content-based image retrieval, population statistics, human–computer interaction, health care, multimedia retrieval systems, demographic collection, and visual surveillance. In this research work, gender classification was carried out using a deep learning approach. A new 64-layer architecture named 4-BSMAB derived from deep AlexNet is proposed. The proposed model was trained on CIFAR-100 dataset utilizing SoftMax classifier. Then, features were obtained from applied datasets with this pre-trained model. The obtained feature set was optimized with ant colony system (ACS) optimization technique. Various classifiers of SVM and KNN were used to perform gender classification utilizing the optimized feature set. Comprehensive experimentation was performed on gender classification datasets, and proposed model produced better results than the existing methods. The suggested model attained highest accuracy, i.e., 85.4%, and 92% AUC on MIT dataset, and best classification results, i.e., 93% accuracy and 96% AUC, on PKU-Reid dataset. The outcomes of extensive experiments carried out on existing standard pedestrian datasets demonstrate that the proposed framework outperformed existing pedestrian gender classification methods, and acceptable results prove the proposed model as a robust model.