A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.
Citations
840 citations
Cites background or methods from "A high-throughput gene knockout pro..."
...crassa high throughput gene knock out program [30] appropriate for transformation of the NJEH deficient strain QM6aΔtmus53 [19]....
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...reesei wild-type DNA and used for yeast mediated recombination with the 3’ and 5’ flanking sequences containing 29 bp homologous overhangs into the shuttle vector pRS426 [30]....
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...We therefore applied the method of yeast mediated recombination for deletion vector construction, which was shown to be highly efficient [30]....
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...Additionally, the possibility to study a group of functionally related genes, such as transcription factors can provide intriguing insights into previously unexplored physiological processes [30,31]....
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...Yeast transformation and preparation was performed essentially as described previously [30,49,50]....
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390 citations
Cites methods from "A high-throughput gene knockout pro..."
...Specifically, APS8, amplified from genomic DNA of Fusarium semitectum using the primer pair aps8-OE-for and aps8-OE-rev, was cloned downstream of the gpd promoter in the restricted pOENAT vector via yeast recombination cloning [114,115]....
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359 citations
294 citations
Cites background from "A high-throughput gene knockout pro..."
...crassa transcription factor deletion set (22) for mutants with deficient growth on cellulose (Avicel) (SI Materials and Methods)....
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...crassa mutant strains was FGSC 2489 (22)....
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279 citations
Cites background from "A high-throughput gene knockout pro..."
...NHEJ-deficient fungal strains have greatly accelerated the development of genome-wide knockouts (3, 11)....
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References
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