A highly optimized DNA vaccine confers complete protective immunity against high-dose lethal lymphocytic choriomeningitis virus challenge.
Devon J. Shedlock,Kendra T. Talbott,Christina Cress,Bernadette Ferraro,Steven Tuyishme,Karthik Mallilankaraman,Neil J. Cisper,Matthew P. Morrow,Stephan J. Wu,Omkar U. Kawalekar,Amir S. Khan,Niranjan Y. Sardesai,Karuppiah Muthumani,Hao Shen,David B. Weiner +14 more
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This is the first non-infectious vaccine conferring complete protective immunity up to 8 weeks after vaccination and demonstrates the potential of 'next-generation' DNA vaccines.About:
This article is published in Vaccine.The article was published on 2011-09-09 and is currently open access. It has received 34 citations till now. The article focuses on the topics: Vaccination & DNA vaccination.read more
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A synthetic consensus anti–spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates
Karuppiah Muthumani,Darryl Falzarano,Emma L. Reuschel,Colleen Tingey,Seleeke Flingai,Daniel O. Villarreal,Megan C. Wise,Ami Patel,Abdullah Izmirly,Abdulelah Aljuaid,Alecia M. Seliga,Geoff Soule,Matthew P. Morrow,Kimberly A. Kraynyak,Amir S. Khan,Dana P. Scott,Friederike Feldmann,Rachel A. LaCasse,Kimberly Meade-White,Atsushi Okumura,Kenneth E. Ugen,Niranjan Y. Sardesai,J. Joseph Kim,Gary P. Kobinger,Heinz Feldmann,David B. Weiner +25 more
TL;DR: It is demonstrated that a consensus MERS spike protein synthetic DNA vaccine can induce protective responses against viral challenge, indicating that this strategy may have value as a possible vaccine modality against this emerging pathogen.
Journal ArticleDOI
Cytomegalovirus Vaccines: Current Status and Future Prospects
TL;DR: An overview of HCMV vaccine candidates in various stages of development is provided, as well as an update on the current status of ongoing clinical trials.
Journal ArticleDOI
Soluble multi-trimeric TNF superfamily ligand adjuvants enhance immune responses to a HIV-1 Gag DNA vaccine
Saravana Kanagavelu,Victoria Snarsky,Victoria Snarsky,James M. Termini,Sachin Gupta,Suzanne Barzee,Suzanne Barzee,Jacqueline A. Wright,Wasif N. Khan,Richard S. Kornbluth,Richard S. Kornbluth,Geoffrey W. Stone +11 more
TL;DR: Evidence is presented that various SP-D-TNFSFL fusion constructs can enhance immune responses following DNA vaccination with HIV-1 Gag expression plasmid, which supports the continued evaluation of SP- D-TN FSFL fusion proteins as molecular adjuvants for DNA and/or viral vector vaccines.
Journal Article
Human cytomegalovirus (HCMV) infection/re-infection: development of a protective HCMV vaccine.
Giuseppe Gerna,Daniele Lilleri +1 more
TL;DR: Although the protective role of cell-mediated immunity has been repeatedly documented, while the protective effect of (mostly neutralizing) antibodies remains partly to be documented, a vaccine stimulating both arms of the immune response would likely confer the highest level of protection against HCMV infection/disease in both H CMV-seronegative and -seropositive individuals.
Journal ArticleDOI
Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine
Jian Yan,Daniel O. Villarreal,Trina Racine,Jaemi S. Chu,Jewell Walters,Matthew P. Morrow,Amir S. Khan,Niranjan Y. Sardesai,J. Joseph Kim,Gary P. Kobinger,David B. Weiner +10 more
TL;DR: This study establishes that this synthetic consensus DNA vaccine represents a new tool for targeting emerging infection, and more importantly, its design, testing and development into seed stock for vaccine production in a few days in the pandemic setting has significant implications for the rapid deployment of vaccines protecting against emerging infectious diseases.
References
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Counting Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during Viral Infection
Kaja Murali-Krishna,John D. Altman,M. Suresh,David J. D. Sourdive,Allan J. Zajac,Joseph D. Miller,Jill E. Slansky,Rafi Ahmed +7 more
TL;DR: Much of the CD8 T cell expansion seen during viral infection represents antigen-specific cells and warrants a revision of current thinking on the size of the antiviral response.
Journal ArticleDOI
Restriction of in vitro T cell-mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system.
TL;DR: Evidence is presented here that the interaction of cytotoxic T cells with other somatic cells budding4–5 lymphocytic choriomeningitis (LCM) virus is similarly restricted.
Journal ArticleDOI
Immunological Memory and Protective Immunity: Understanding Their Relation
Rafi Ahmed,David Gray +1 more
TL;DR: The current understanding of the cellular basis of immune memory is reviewed and the relative contributions made to protective immunity by memory and effector T and B cells are examined.
Journal ArticleDOI
Requirement for CD4 T cell help in generating functional CD8 T cell memory
Devon J. Shedlock,Hao Shen +1 more
TL;DR: Results demonstrate a previously undescribed role for CD4 help in the development of functional CD8 memory and show that depletion of CD4 cells during the recall response has minimal effect, whereas depletion during the priming phase leads to reduced responses to reinfection.
Journal ArticleDOI
Massive Expansion of Antigen-Specific CD8+ T Cells during an Acute Virus Infection
Eric A. Butz,Michael J. Bevan +1 more
TL;DR: Analysis of the effect of the monospecific TCR-transgenic T cells on the host response to three LCMV epitopes suggests that CTL precursors compete for sites on the APC in an epitope-specific fashion and that this competition determines the specificity of the response.
Related Papers (5)
A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates.
Karthik Mallilankaraman,Devon J. Shedlock,Huihui Bao,Omkar U. Kawalekar,Paolo Fagone,Aarthi A. Ramanathan,Bernadette Ferraro,Jennifer Stabenow,Paluru Vijayachari,Senthil G. Sundaram,N. Muruganandam,Gopalsamy Sarangan,Padma Srikanth,Amir S. Khan,Mark G. Lewis,J. Joseph Kim,Niranjan Y. Sardesai,Karuppiah Muthumani,David B. Weiner +18 more