A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
Alekos Simoni,Andrew Hammond,Andrew Hammond,Andrea Beaghton,Roberto Galizi,Roberto Galizi,Chrysanthi Taxiarchi,Kyros Kyrou,Dario Meacci,Matthew Gribble,Giulia Morselli,Austin Burt,Tony Nolan,Tony Nolan,Andrea Crisanti,Andrea Crisanti +15 more
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TLDR
A male-biased sex-distorter gene drive (SDGD) is reported in the human malaria vector Anopheles gambiae and is predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility.Abstract:
Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10–14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.read more
Citations
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Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi.
Adriana Adolfi,Adriana Adolfi,Valentino M. Gantz,Nijole Jasinskiene,H. K. Lee,Kristy S. Hwang,Gerard Terradas,Emily A. Bulger,Arunachalam Ramaiah,Jared B. Bennett,J. J. Emerson,John M. Marshall,Ethan Bier,Anthony A. James +13 more
TL;DR: A recoded gene-drive rescue system for population modification of the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function is developed.
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Modelling the suppression of a malaria vector using a CRISPR-Cas9 gene drive to reduce female fertility
TL;DR: Simulation modelling indicates that considerable suppression of vector populations can be achieved within a few years of using a female sterility gene drive, though the impact is likely to be heterogeneous in space and time.
Journal ArticleDOI
Core commitments for field trials of gene drive organisms.
Kanya C. Long,Luke Alphey,George J. Annas,Cinnamon S. Bloss,Karl J. Campbell,Jackson Champer,Chun-Hong Chen,Amit Choudhary,George M. Church,James P. Collins,Kimberly L. Cooper,Jason A. Delborne,Owain R. Edwards,Claudia Emerson,Kevin M. Esvelt,Sam Weiss Evans,Robert M. Friedman,Valentino M. Gantz,Fred Gould,Sarah Hartley,Elizabeth Heitman,Janet Hemingway,Hirotaka Kanuka,Jennifer Kuzma,James V. Lavery,Yoosook Lee,Marcé D. Lorenzen,Jeantine E. Lunshof,John M. Marshall,Philipp W. Messer,Craig Montell,Kenneth A. Oye,Megan J. Palmer,Philippos Aris Papathanos,Prasad N. Paradkar,Antoinette J. Piaggio,Jason L. Rasgon,Gordana Rašić,Larisa Rudenko,J. Royden Saah,Maxwell J. Scott,Jolene T. Sutton,Adam E. Vorsino,Omar S. Akbari +43 more
TL;DR: Significant commitments are outlined that are deemed critical for responsible conduct of a field trial and to ensure that these technologies, if they are introduced, serve the public interest.
Journal ArticleDOI
Combating mosquito-borne diseases using genetic control technologies.
Guan-Hong Wang,Guan-Hong Wang,Stephanie Gamez,Robyn R. Raban,John M. Marshall,Luke Alphey,Ming Li,Jason L. Rasgon,Omar S. Akbari +8 more
TL;DR: A review of the latest developments, notable similarities, and critical distinctions between these promising technologies and discuss their future applications for mosquito-borne disease control can be found in this paper, where the authors discuss the future applications of these technologies.
Journal ArticleDOI
Nix alone is sufficient to convert female Aedes aegypti into fertile males and myo-sex is needed for male flight.
Azadeh Aryan,Michelle A. E. Anderson,James K. Biedler,Yumin Qi,Justin M. Overcash,Anastasia N. Naumenko,Maria V. Sharakhova,Maria V. Sharakhova,Chunhong Mao,Zach N. Adelman,Zhijian Tu +10 more
TL;DR: The Nix transgene alone, in the absence of the M-locus, was sufficient to convert females into males with all male-specific sexually dimorphic features and male-like gene expression and Nix-mediated female-to-male conversion was 100% penetrant and stable over many generations.
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A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae
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