Journal ArticleDOI
A marine microbial consortium apparently mediating anaerobic oxidation of methane
Antje Boetius,Katrin Ravenschlag,Carsten J. Schubert,Dirk Rickert,Friedrich Widdel,Armin Gieseke,Rudolf Amann,Bo Barker Jørgensen,Ursula Witte,Olaf Pfannkuche +9 more
Reads0
Chats0
TLDR
In this article, the authors provide microscopic evidence for a structured consortium of archaea and sulphate-reducing bacteria, which are identified by fluorescence in situ hybridization using specific 16S rRNA-targeted oligonucleotide probes.Abstract:
A large fraction of globally produced methane is converted to CO2 by anaerobic oxidation in marine sediments. Strong geochemical evidence for net methane consumption in anoxic sediments is based on methane profiles, radiotracer experiments and stable carbon isotope data. But the elusive microorganisms mediating this reaction have not yet been isolated, and the pathway of anaerobic oxidation of methane is insufficiently understood. Recent data suggest that certain archaea reverse the process of methanogenesis by interaction with sulphate-reducing bacteria. Here we provide microscopic evidence for a structured consortium of archaea and sulphate-reducing bacteria, which we identified by fluorescence in situ hybridization using specific 16S rRNA-targeted oligonucleotide probes. In this example of a structured archaeal-bacterial symbiosis, the archaea grow in dense aggregates of about 100 cells and are surrounded by sulphate-reducing bacteria. These aggregates were abundant in gas-hydrate-rich sediments with extremely high rates of methane-based sulphate reduction, and apparently mediate anaerobic oxidation of methane.read more
Citations
More filters
MonographDOI
The Biomarker Guide
TL;DR: The second edition of The Biomarker Guide as mentioned in this paper provides a comprehensive account of the role that biomarker technology plays both in petroleum exploration and in understanding Earth history and processes.
Journal ArticleDOI
The Uncultured Microbial Majority
TL;DR: Genome sequence information that would allow ribosomal RNA gene trees to be related to broader patterns in microbial genome evolution is scant, and therefore microbial diversity remains largely unexplored territory.
Journal ArticleDOI
The ecology and biotechnology of sulphate-reducing bacteria
Gerard Muyzer,Alfons J. M. Stams +1 more
TL;DR: Sulphate-reducing bacteria are anaerobic microorganisms that use sulphate as a terminal electron acceptor in, for example, the degradation of organic compounds, and are ubiquitous in anoxic habitats.
Journal ArticleDOI
Biodiversity on the Rocks: Macrofauna Inhabiting Authigenic Carbonate at Costa Rica Methane Seeps.
Lisa A. Levin,Guillermo Mendoza,Benjamin M. Grupe,Jennifer P. Gonzalez,Brittany M. Jellison,Greg W. Rouse,Andrew R. Thurber,Anders Warén +7 more
TL;DR: The substrate and nutritional heterogeneity introduced by authigenic seep carbonates act to promote diverse, uniquely adapted assemblages, even after seepage ceases, demonstrating the significant role of carbonate rocks in promoting diversity.
Journal ArticleDOI
The snowball Earth hypothesis: testing the limits of global change
Paul Hoffman,Daniel P. Schrag +1 more
TL;DR: The recent discovery that late Neoproterozoic ice sheets extended to sea level near the equator poses a palaeoenvironmental conundrum as discussed by the authors, which does not account for major features such as abrupt onsets and terminations of discrete glacial events, their close association with large (> 10&) negative d 13 C shifts in seawater proxies, the deposition of strange carbonate layers (cap carbonates) globally during postglacial sea-level rise, and the return of large sedimentary iron formations.
References
More filters
Journal ArticleDOI
Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
TL;DR: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.
Journal ArticleDOI
Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
TL;DR: Fluorescent-dye-conjugated oligonucleotides were used to classify 14 Fibrobacter strains by fluorescence microscopy and the direct detection of F. intestinalis in mouse cecum samples demonstrated the application of this technique to the characterization of complex natural samples.
Journal ArticleDOI
Methane-consuming archaebacteria in marine sediments
TL;DR: Studies of sediments related to a decomposing methane hydrate provide strong evidence that methane is being consumed by archaebacteria that are phylogenetically distinct from known methanogens.
Journal ArticleDOI
Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria.
TL;DR: Volume 62, no. 6, p. 2157, Table 1: the sequence for probe Nso1225, 5(prm1)-CGCGATTGTATTACGTGTGTGA-3( prm1), should read 5(PRm1-CGCCATTGTattACGT GTGA- 3(prM1).
Related Papers (5)
Anaerobic Oxidation of Methane: Progress with an Unknown Process
Katrin Knittel,Antje Boetius +1 more