Journal ArticleDOI
A method for the determination of dATP and dTTP in picomole amounts.
Uno Lindberg,Lambert Skoog +1 more
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This procedure is a modification of an enzymic assay introduced by Solter and Handsehumacher and is approximately 100 times more sensitive and successfully used to measure dATP and dTTP contents of cultured cells and have found no inhibitors of the polymerization reaction in such extracts.About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 191 citations till now.read more
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Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters : Evaluation and field results
Jed A. Fuhrman,Farooq Azam +1 more
TL;DR: To assess bacterioplankton production in the sea, a procedure for measuring growth based on incorporation of tritiated thymidine into DNA is developed; the accuracy of this procedure was tested under a variety of laboratory and field conditions.
Journal ArticleDOI
Effects of thymidine on deoxyribonucleoside triphosphate pools and deoxyribonucleic acid synthesis in Chinese hamster ovary cells.
Gunnar Bjursell,Peter Reichard +1 more
TL;DR: The results suggest the possibility that the size of the dCTP pool may have a regulatory function for the rate of DNA synthesis, and show that allosteric mechanisms shown with a purified enzyme (ribonucleoside diphosphate reductase) operate in intact cells.
Journal ArticleDOI
Effects of hydroxyurea and 1-beta-D-arabinofuranosyl-cytosine on deoxyribonucleotide pools in mouse embryo cells.
Lambert Skoog,Bo Nordenskjöld +1 more
TL;DR: The results show that the inhibition of DNA synthesis by hydroxyurea is correlated with diminishing pools of dGTP and dATP and a decreased turnover of dTTP, indicating a generally decreased deoxyribonucleotide formation presumably due to the inhibited ribonucleoside-diphosphate reductase.
Journal ArticleDOI
A rapid and sensitive high pressure liquid chromatography assay for deoxyribonucleoside triphosphates in cell extracts.
TL;DR: An assay for simultaneous quantitation of deoxyribonucleoside triphosphates in cell extracts is described, which offers a simple and rapid alternative to either DNA polymerase or thin-layer chromatography assays for dNTPs in cellular extracts.
Journal ArticleDOI
High glucose induces DNA damage in cultured human endothelial cells.
TL;DR: It is suggested that, under conditions of high ambient glucose, excess glucose entry in cells that are insulin independent for glucose transport may, directly or indirectly, perturb DNA function and the possibility that different individual capabilities to repair DNA damage may represent a mechanism for different individual susceptibilities to development of diabetic vascular complication.
References
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A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
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Enzymatic Synthesis of Deoxyribonucleic Acid I. PREPARATION OF SUBSTRATES AND PARTIAL PURIFICATION OF AN ENZYME FROM ESCHERICHIA COLI
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Enzymatic Synthesis of Deoxyribonucleic Acid. IX. The Polymerase Formed after T2 Bacteriophage Infection of Escherichia coli: A New Enzyme
TL;DR: Bello, Van Bibber, and Bessman have shown that the deoxyguanylate kinase of infected cells is physically distinct and separable from the host cell enzyme, and the polymerase of uninfected E. coli (E. coli polymerase) and the T2 polymerase are distinct and different enzymes.
Journal ArticleDOI
A deoxyribonucleic acid phosphatase-exonuclease from escherichia coli. ii. characterization of the exonuclease activity.
TL;DR: The specificity of the enzyme in attacking native double stranded DNA and its inability to act on small polynucleotides make it a unique and useful reagent in studies of DN-4 structure and metabolism.
Related Papers (5)
A rapid quantitative determination of deoxyribonucleoside triphosphates based on the enzymatic synthesis of DNA.
Effects of hydroxyurea and 1-beta-D-arabinofuranosyl-cytosine on deoxyribonucleotide pools in mouse embryo cells.
Lambert Skoog,Bo Nordenskjöld +1 more