A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Citations
673 citations
Cites background from "A Multipurpose Toolkit to Enable Ad..."
...With the flexibility and expandability of CRISPR/Cas9 (Cermak et al., 2017), our approach can produce hundreds of regulatory mutations to systematically assess the association of cis-regulatory regions with phenotypic variation by dissecting the functions of specific CREs, the modules in which they…...
[...]
...However, based on previouswork, we hypothesized that multiple elements of CRISPR/Cas9 technology could be integrated to engineer diverse types and strengths of cis-regulatory mutations (Cermak et al., 2017; Soyk et al., 2017; Swinnen et al., 2016)....
[...]
...With the flexibility and expandability of CRISPR/Cas9 (Cermak et al., 2017), our approach can produce hundreds of regulatory mutations to systematically assess the association of cis-regulatory regions with phenotypic variation by dissecting the functions of specific CREs, the modules in which they function, and their spatial organization....
[...]
...With the remarkable pace that genomeediting andplant transformation technologies are advancing (Barrangou and Doudna, 2016; Cermak et al., 2017; Lowe et al., 2016), expansive libraries of regulatory alleles could soon be created in both plants and animals (Van Eenennaam, 2017)....
[...]
478 citations
463 citations
381 citations
338 citations
References
44,587 citations
"A Multipurpose Toolkit to Enable Ad..." refers methods in this paper
...Gel images were analyzed using ImageJ (Schneider et al., 2012)....
[...]
...quantified by image analysis using ImageJ (Schneider et al., 2012)....
[...]
...Whole leaves were scanned and the stained area was quantified by image analysis using ImageJ (Schneider et al., 2012)....
[...]
...NIH Image to ImageJ: 25 years of image analysis....
[...]
12,865 citations
"A Multipurpose Toolkit to Enable Ad..." refers background in this paper
...Inour experiments, precise ligation of Cas9-cleaved DNA without the intervening sequence would result in a deletion of 85 bp. Gain or loss of nucleotides due to staggered Cas9 cleavage (Jinek et al., 2012; Li et al., 2015) and/or exonuclease activity could result in shorter or longer deletions....
[...]
...Gain or loss of nucleotides due to staggered Cas9 cleavage (Jinek et al., 2012; Li et al., 2015) and/or exonuclease activity could result in shorter or longer deletions....
[...]
12,265 citations
10,746 citations
"A Multipurpose Toolkit to Enable Ad..." refers background in this paper
...Singlenickasesdonot inducedetectable levels of on-targetNHEJ-mediatedmutagenesis (Cong et al., 2013; Ran et al., 2013; Fauser et al., 2014), whereas paired nickases, which create two adjacent nicks in the target sequence, induce mutations at efficiencies comparable to native Cas9 (Ran et al....
[...]
...Singlenickasesdonot inducedetectable levels of on-targetNHEJ-mediatedmutagenesis (Cong et al., 2013; Ran et al., 2013; Fauser et al., 2014), whereas paired nickases, which create two adjacent nicks in the target sequence, induce mutations at efficiencies comparable to native Cas9 (Ran et al., 2013;…...
[...]
8,117 citations
"A Multipurpose Toolkit to Enable Ad..." refers methods in this paper
...Direct TALEN cloning vectors pDIRECT_37-39J were first constructed by Gibson assembly (Gibson et al., 2009) of PCRproducts containing parts of pCLEAN-G126 backbone (Thole et al....
[...]
...Direct TALEN cloning vectors pDIRECT_37-39J were first constructed by Gibson assembly (Gibson et al., 2009) of PCRproducts containing parts of pCLEAN-G126 backbone (Thole et al., 2007; GenBank accession no....
[...]