A Naphthalimide-Based Fluorescent Probe for the Detection and Imaging of Mercury Ions in Living Cells
TL;DR: In this paper, a 1,8-naphthalimide-based fluorescent probe NADP for the detection of Hg2+ based on a fluorescence enhancement strategy has been designed and synthesized.
Abstract: The selective and efficient monitoring of mercury (Hg2+ ) contamination found in the environment and ecosystem has been carried out. Thus, a new 1,8-naphthalimide-based fluorescent probe NADP for the detection of Hg2+ based on a fluorescence enhancement strategy has been designed and synthesized. The NADP probe can detect Hg2+ with high selectivity and sensitivity and a low detection limit of 13 nm. The detection mechanism was based on a Hg2+ -triggered deprotection reaction, resulting in a dramatic change in fluorescence from colorless to green at physiological pH. Most importantly, biological investigation has shown that the NADP probe can be successfully applied to the monitoring of Hg2+ in living cells and zebrafish with low cytotoxicity.
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TL;DR: In this article , two photo-crosslinked membranes were obtained by copolymerization of the N-vinylpyrrolidone, butyl acrylate and ethyl methacrylate monomers functionalized with naphthalimide groups, as pH sensitive fluorescence probes.
Abstract: Novel water-swollen photo-crosslinked membranes were obtained by copolymerization of the N-vinylpyrrolidone, butyl acrylate and ethyl methacrylate monomers functionalized with naphthalimide groups, as pH sensitive fluorescence probes. For that purpose, two monomers with pending naphthalimide groups anchored to ethyl methacrylate through alkyl chains with different length, were previously synthesized. The membranes were characterized using different techniques. The pH dependence of absorbance and the corresponding quenching of fluorescence were investigated and related to the structure of naphthalimide substituents linked to the membrane. The new solid sensors exhibited sensitive fluorescence changes at pH < 3, and lower time response was determined for membranes where the sensing group was linked through longer alkyl chain to the polymer matrix. The membranes were solid, thermally stable and easily handled to be applied as sensor materials, and showed a reversible behavior, which is an important feature for further fabrication of an economical on-site tool for the acidity detection in aqueous environments.
3 citations
TL;DR: Mercury (Hg), as the only heavy metal that can complete the cycle in the biosphere, can further accumulate in the human body through the food chain, causing irreversible damage to the body.
Abstract: Mercury (Hg), as the only heavy metal that can complete the cycle in the biosphere, can further accumulate in the human body through the food chain, causing irreversible damage to...
2 citations
01 Mar 2023
TL;DR: In this article , a fluorescent probe called 4-(1, 3-dithian-2-yl)-1, 1,1-diphenyl- λ5-phosphinine-2,6-dicarbonitrile (DDPD) with optical behavior was used to detect Hg2+ ions in organic semi-aqueous solutions.
Abstract: Due to its great toxicity to the bio-aquatic environment, developing highly selective and sensitive probes for the detection of mercury ions in solutions as well as in living cells is a major problem. To detect Hg2+ ions in organic semi-aqueous solutions, we created a fluorescent probe called 4-(1, 3-dithian-2-yl)-1,1-diphenyl- λ5-phosphinine-2,6-dicarbonitrile (DDPD) with optical behaviour. Through the Hg2+-promoted deprotection process of thioacetals, which was followed by considerable changes in fluorescence colour and spectral pattern, the probe DDPD selectively detected Hg2+ ions in a broad pH range with a detection limit of 56 nM. Specifically, DDPD can be used to qualitatively identify Hg2+ ions using test strips made of Whatman filter paper and also in HeLa cells it can identify Hg2+ ions and image them using fluorescent microscopy.
1 citations
TL;DR: In this paper , a simple, low-cost, eco-benign, and portable naked-eye solid-state sensor for the selective and sensitive colorimetric detection and recovery of ultra-trace concentrations of Pb(II), Hg(II) and Cd(II).
TL;DR: In this article , a photopolymer resin is developed, in which a visible light photoinitiator (fluorinated diphenyl titanocene) with a broad absorption and an optical absorber (naphthalimide class dye, maximum absorption at 445 nm) are carefully matched with the upconversion emissions of lanthanide-doped phosphors (both micron-sized phosphors and customized core/shell/shell nanoparticles).
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TL;DR: Five practical examples involving a wide variety of systems and analysis methods are given to illustrate the usefulness of Multiwfn, a multifunctional program for wavefunction analysis.
Abstract: Multiwfn is a multifunctional program for wavefunction analysis. Its main functions are: (1) Calculating and visualizing real space function, such as electrostatic potential and electron localization function at point, in a line, in a plane or in a spatial scope. (2) Population analysis. (3) Bond order analysis. (4) Orbital composition analysis. (5) Plot density-of-states and spectrum. (6) Topology analysis for electron density. Some other useful utilities involved in quantum chemistry studies are also provided. The built-in graph module enables the results of wavefunction analysis to be plotted directly or exported to high-quality graphic file. The program interface is very user-friendly and suitable for both research and teaching purpose. The code of Multiwfn is substantially optimized and parallelized. Its efficiency is demonstrated to be significantly higher than related programs with the same functions. Five practical examples involving a wide variety of systems and analysis methods are given to illustrate the usefulness of Multiwfn. The program is free of charge and open-source. Its precompiled file and source codes are available from http://multiwfn.codeplex.com.
17,273 citations
TL;DR: An expanded porphyrin [26]hexaphyrin (1.1.2.1) was exploited as a fluorescent chemodosimeter for Ag + ions with high sensitivity and selectivity via near-infrared luminescence above 900 nm, a region that is free from optical interference in the visible wavelength range induced by the commonly used matrix and other organic compounds.
Abstract: An expanded porphyrin [26]hexaphyrin(1.1.1.1.1.1) was exploited as a fluorescent chemodosimeter for Ag + ions with high sensitivity and selectivity via near-infrared luminescence above 900 nm, a region that is free from optical interference in the visible wavelength range induced by the commonly used matrix and other organic compounds. The association constant for the Ag + –porphyrin complexation was evaluated by spectroscopic titration method to be 7.24 10 10 M 1 .
245 citations
TL;DR: In this review, recent progress in the development of gold nanoparticle-based colorimetric assays for Hg(2+) is summarized, with a particular emphasis on examples of functionalized gold nanoparticles systems with oligonucleotides, oligopeptides, and functional molecules.
Abstract: The development of rapid, specific, cost-effective, and robust tools in monitoring Hg2+ levels in both environmental and biological samples is of utmost importance due to the severe mercury toxicity to humans. A number of techniques exist, but the colorimetric assay, which is reviewed herein, is shown to be a possible tool in monitoring the level of mercury. These assays allow transforming target sensing events into color changes, which have applicable potential for in-the-field application through naked-eye detection. Specifically, plasmonic nanoparticle-based colorimetric assay exhibits a much better propensity for identifying various targets in terms of sensitivity, solubility, and stability compared to commonly used organic chromophores. In this review, recent progress in the development of gold nanoparticle-based colorimetric assays for Hg2+ is summarized, with a particular emphasis on examples of functionalized gold nanoparticle systems with oligonucleotides, oligopeptides, and functional molecules. Besides highlighting the current design principle for plasmonic nanoparticle-based colorimetric probes, the discussions on challenges and the prospect of next-generation probes for in-the-field applications are also presented.
244 citations
TL;DR: A plausible link among Hg levels, its chemical speciation and phytoavailability in soil, accumulation in plants, phytotoxicity and detoxification of Hg inside the plant is traced and well summarized and up-to-date data are described.
149 citations
TL;DR: A new "turn-on" fluorescent probe (1-CN) was obtained based on the deprotection reaction of the dithioacetal promoted by Hg2+ ions, which could sense mercury ions sensitively and selectively, with the detection limit of 8×10(-7) M.
Abstract: By intelligent design, a new “turn-on” fluorescent probe (1-CN) was obtained based on the deprotection reaction of the dithioacetal promoted by Hg2+ ions, which could sense mercury ions sensitively and selectively, with the detection limit of 8 × 10–7 M. Thanks to the apparent turn-on signal, 1-CN has been successfully applied to rapidly detect trace amounts of mercury ions as test strips and cell image.
109 citations