A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
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TLDR
The rd29A promoter in both transgenic Arabidopsis and tobacco was analyzed and a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, was identified that is involved in the first rapid response of rd 29A to conditions of dehydration or high salt.Abstract:
Two genes, rd29A and rd29B, which are closely located on the Arabidopsis genome, are differentially induced under conditions of dehydration, low temperature, high salt, or treatment with exogenous abscisic acid (ABA). It appears that rd29A has at least two cis-acting elements, one involved in the ABA-associated response to dehydration and the other induced by changes in osmotic potential, and that rd29B contains at least one cis-acting element that is involved in ABA-responsive, slow induction. We analyzed the rd29A promoter in both transgenic Arabidopsis and tobacco and identified a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, that is involved in the first rapid response of rd29A to conditions of dehydration or high salt. DRE is also involved in the induction by low temperature but does not function in the ABA-responsive, slow expression of rd29A. Nuclear proteins that specifically bind to DRE were detected in Arabidopsis plants under either high-salt or normal conditions. Different cis-acting elements seem to function in the two-step induction of rd29A and in the slow induction of rd29B under conditions of dehydration, high salt, or low temperature.read more
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Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
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Gene expression in response to abscisic acid and osmotic stress.
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TL;DR: Several ABA-responsive genes have been isolated and a major goal of the research discussed below is to understand the role these genes play in osmotic stress and desiccation tolerance.
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Leon Dure,Martha L. Crouch,John J. Harada,Tuan-Hua David Ho,John Mundy,Ralph S. Quatrano,Terry L. Thomas,Zinmay Renee Sung +7 more
TL;DR: Analysis of conserved tracts of amino acid sequence among LEA proteins from several species suggests that it exists as an amphiphilic α helix which may serve as the basis for higher order structure.