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Journal ArticleDOI: 10.1039/D1NR00244A

A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core-shell type magnetic nanoparticles.

04 Mar 2021-Nanoscale (Royal Society of Chemistry (RSC))-Vol. 13, Iss: 8, pp 4660-4669
Abstract: Lung cancer is one of deadliest and most life threatening cancer types. Cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) is a significant biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Due to these reasons, a novel electrochemical immunosensor based on a silicon nitride (Si3N4)–molybdenum disulfide (MoS2) composite on multi-walled carbon nanotubes (Si3N4/MoS2–MWCNTs) as an electrochemical sensor platform and core–shell type magnetic mesoporous silica nanoparticles@gold nanoparticles (MMSNs@AuNPs) as a signal amplifier was presented for CYFRA21-1 detection in this study. Capture antibody (Ab1) immobilization on a Si3N4/MoS2–MWCNT modified glassy carbon electrode (Si3N4/MoS2–MWCNTs/GCE) was firstly successfully performed by stable electrostatic/ionic interactions between the –NH2 groups of the capture antibody and the polar groups of Si3N4/MoS2. Then, specific antibody–antigen interactions between the electrochemical sensor platform and the signal amplifier formed a novel voltammetric CYFRA21-1 immunosensor. The prepared composite materials and electrochemical sensor surfaces were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01–1.0 pg mL−1 and a low detection limit (LOD) of 2.00 fg mL−1 were also obtained for analytical applications. Thus, the proposed immunosensor based on Si3N4/MoS2–MWCNTs and MMSNs@AuNPs has great potential for medical diagnosis of lung cancer.

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14 results found


Open accessJournal ArticleDOI: 10.1016/J.BIOACTMAT.2021.04.021
Jianling Wang1, Lihua Sui1, Jia Huang1, Lu Miao1  +7 moreInstitutions (2)
Abstract: Molybdenum is a trace dietary element necessary for the survival of humans. Some molybdenum-bearing enzymes are involved in key metabolic activities in the human body (such as xanthine oxidase, aldehyde oxidase and sulfite oxidase). Many molybdenum-based compounds have been widely used in biomedical research. Especially, MoS2-nanomaterials have attracted more attention in cancer diagnosis and treatment recently because of their unique physical and chemical properties. MoS2 can adsorb various biomolecules and drug molecules via covalent or non-covalent interactions because it is easy to modify and possess a high specific surface area, improving its tumor targeting and colloidal stability, as well as accuracy and sensitivity for detecting specific biomarkers. At the same time, in the near-infrared (NIR) window, MoS2 has excellent optical absorption and prominent photothermal conversion efficiency, which can achieve NIR-based phototherapy and NIR-responsive controlled drug-release. Significantly, the modified MoS2-nanocomposite can specifically respond to the tumor microenvironment, leading to drug accumulation in the tumor site increased, reducing its side effects on non-cancerous tissues, and improved therapeutic effect. In this review, we introduced the latest developments of MoS2-nanocomposites in cancer diagnosis and therapy, mainly focusing on biosensors, bioimaging, chemotherapy, phototherapy, microwave hyperthermia, and combination therapy. Furthermore, we also discuss the current challenges and prospects of MoS2-nanocomposites in cancer treatment.

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15 Citations


Journal ArticleDOI: 10.1016/J.CDC.2021.100718
Abstract: Acetaminophen (AC) is one of the most commonly consumed painkillers worldwide and hence its increased use leads to higher concentrations in source water, thus needful its analysis. The voltammetric analysis of AC using the fabricated sensor was presented in this article. The voltammetric sensor based on the poly asparagine modified carbon nanotube and graphene mixed paste electrode (PASMCNTMGPE) was constructed. The constructed electrode unveiled a boosted electrocatalytic activity towards the detection of AC than the unmodified electrode through the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. The Field Emission Scanning Electron Microscopy (FE-SEM) was utilized to verify the morphological feature of the designed electrode. The charge transfer resistance of the designed electrode was studied by electrochemical impedance spectroscopy (EIS). Some of the factors influencing the voltammetric signal of AC such as the number of electropolymerisation cycles and pH were optimized. The redox reaction of AC at the PASMCNTMGPE is a pH-dependent and adsorption-controlled process. The designed electrode yielded a low detection limit (4.10 × 10−8 M) for AC. In addition, the prepared electrode demonstrated an excellent anti-fouling property, reliability, selectivity, and repeatability. More importantly, the feasibility of the constructed sensor was evaluated by analyzing AC in the pharmaceutical and biological samples.

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2 Citations



Journal ArticleDOI: 10.1007/S00604-021-05005-7
Rabia Ashiq1, Batool Fatima1, Mohibullah Shah1, Dilshad Hussain2  +6 moreInstitutions (4)
12 Sep 2021-Mikrochimica Acta
Abstract: An electrochemical sensor based on an antimony/nitrogen-doped porous carbon (Sb/NPC) composite has been developed for the quantitative detection of albumin from hepatocellular carcinoma (HCC) patients. Sb/NPC is hydrothermally synthesized from Sn/NPC precursors. The synthesized precursor (Sn/NPC) and the product (Sb/NPC) are characterized by XRD, FTIR, TGA, UV/Vis, SEM, and AFM. Cyclic voltammetry, chronoamperometry, and electrochemical impedance studies are used to investigate the electrochemical performance of Sb/NPC-GCE. Sb/NPC-GCE detects albumin at physiological pH of 7.4 in the potential range 0.92 V and 0.09 V for oxidation and reduction, respectively. LOD and recovery of Sb/NPC-GCE for the determination of albumin are 0.13 ng.mL−1 and 66.6 ± 0.97–100 ± 2.73%, respectively. Chronoamperometry of the modified working electrode demonstrates its stability for 14 h, indicating its reusability and reproducibility. Sb/NPC-GCE is a selective sensor for albumin detection in the presence of interfering species. The electrode has been applied for albumin detection in human serum samples of HCC patients. A negative correlation of albumin with alpha-fetoprotein levels in HCC patients is observed by statistical analysis.

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Topics: Chronoamperometry (51%)

1 Citations


Journal ArticleDOI: 10.1016/J.BIOS.2021.113529
Myeongsoon Lee1, Hak Jun Kim1, Don Kim1Institutions (1)
Abstract: Microcystin-LR (MC-LR) is a cyanobacterial toxin produced as a result of eutrophication in polluted water in warm weather conditions. The MC-LR could cause health problems in mammal organs such as the liver, heart, and muscle. Therefore, the World Health Organization (WHO) has stipulated a limit of

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References
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57 results found


Journal ArticleDOI: 10.1021/NN102592H
Weijie Luo1, Changfeng Zhu1, Shao Su1, Di Li1  +3 moreInstitutions (1)
30 Nov 2010-ACS Nano
Abstract: Size and shape of nanoparticles are generally controlled by external influence factors such as reaction temperature, time, precursor, and/or surfactant concentration. Lack of external influence may eventually lead to unregulated growth of nanoparticles and possibly loss of their nanoscale properties. Here we report a gold nanoparticle (AuNPs)-based self-catalyzed and self-limiting system that exploits the glucose oxidase-like catalytic activity of AuNPs. We find that the AuNP-catalyzed glucose oxidation in situ produces hydrogen peroxide (H(2)O(2)) that induces the AuNPs seeded growth in the presence of chloroauric add (HAuCl(4)). This crystal growth of AuNPs is internally regulated via two negative feedback factors, size-dependent activity decrease of AuNPs and product (gluconic acid)-induced surface passivation, leading to a rapidly self-limiting system. Interestingly, the size, shape, and catalytic activities of AuNPs are simultaneously controlled in this system. We expect that it provides a new method for controlled synthesis of novel nanomaterials, design of "smart" self-limiting nanomedicine, as well as in-depth understanding of self-limiting systems in nature.

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Topics: Glucose oxidase (53%), Colloidal gold (52%)

357 Citations


Journal ArticleDOI: 10.1016/J.ACA.2012.10.060
Xiaomei Pei1, Bing Zhang1, Juan Tang1, Bingqian Liu1  +2 moreInstitutions (1)
Abstract: Methods based on sandwich-type immunosensors and immunoassays have been developed for detection of multivalent antigens/analytes with more than one eptiope due to the use of two matched antibodies. High-affinity antibodies and appropriate labels are usually employed for the amplification of detectable signal. Recent research has looked to develop innovative and powerful novel nanoparticle labels, controlling and tailoring their properties in a very predictable manner to meet the requirements of specific applications. This articles reviews recent advances, exploiting nanoparticle labels, in the sandwich-type immunosensors and immunoassays. Routine approaches involve noble metal nanoparticles, carbon nanomaterials, semiconductor nanoparticles, metal oxide nanostructures, and hybrid nanostructures. The enormous signal enhancement associated with the use of nanoparticle labels and with the formation of nanoparticle-antibody-antigen assemblies provides the basis for sensitive detection of disease-related proteins or biomolecules. Techniques commonly rely on the use of biofunctionalized nanoparticles, inorganic-biological hybrid nanoparticles, and signal tag-doped nanoparticles. Rather than being exhaustive, this review focuses on selected examples to illustrate novel concepts and promising applications. Approaches described include the biofunctionalized nanoparticles, inorganic-biological hybrid nanoparticles, and signal tage-doped nanoparticles. Further, promising application in electrochemical, mass-sensitive, optical and multianalyte detection are discussed in detail.

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322 Citations


Journal ArticleDOI: 10.1039/C3CS60077G
Xiliang Luo1, Jason J. Davis2Institutions (2)
Abstract: Electrical detection methodologies are likely to underpin the progressive drive towards miniaturised, sensitive and portable biomarker detection protocols. In being easily integrated within standard electronic microfabrication formats, and developing capability in microfluidics, the facile multiplexed detection of a range of proteins in a small analytical volume becomes entirely feasible with something costing just a few thousand pounds and benchtop or handheld in scale. In this review, we focus on recent important advances in label free assays of protein using a number of electrical methods, including those based on electrochemical impedance spectroscopy (EIS), amperometry/voltammetry, potentiometry, conductometry and field-effect methods. We introduce their mechanistic features and examples of application and sensitivity. The current state of the art, real world applications and challenges are outlined.

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303 Citations


Journal ArticleDOI: 10.1021/JA9010157
Sarah Angelos1, Niveen M. Khashab2, Ying-Wei Yang3, Ying-Wei Yang1  +4 moreInstitutions (3)
Abstract: Mechanized nanoparticles (MNPs) consisting of supramolecular machines attached to the surface of mesoporous silica nanoparticles are designed to release encapsulated guest molecules controllably under pH activation. The molecular machines are comprised of cucurbit[6]uril (CB[6]) rings that encircle tethered trisammonium stalks and can be tuned to respond under specific pH conditions through chemical modification of the stalks. Luminescence spectroscopy demonstrates that the MNPs are able to contain guest molecules within nanopores at neutral pH levels and then release them once the pH is lowered or raised.

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Topics: Mesoporous silica (53%), Molecular switch (51%)

299 Citations


Journal ArticleDOI: 10.1016/J.JCIS.2019.10.007
Abstract: In this article, we report the simultaneous determination cholesterol (CL), ascorbic (AA) acid and uric acid (UA) at a carbon paste electrode (CPE) modified with copper oxide decorated reduced graphene (CuO-rGR), with 1-methyl-3-octylimidazolium tetrafluoroborate (1M3OIDTFB) as a binder. The electrode, CuO-rGR/1M3OIDTFB/CPE, showed remarkable sensitivities towards the determination of the analytes, and well defined and clearly separated oxidation peaks were obtained during their simultaneous analysis in a buffer solution at pH 7.4. The differences observed between their peaks potentials are as follows: 430 mV (between CL and AA), 270 mV (between AA and UA) and 700 mV (between CL and UA). The morphologies and structure properties of the CuO-rGR were investigated by FESEMD and EDAX methods. The CuO-rGR/1M3OIDTFB/CPE displayed linear response in the concentration ranges 0.04–300.0 μM, 0.04–240.0 μM and 0.4–400.0 μM for CL, AA and UA with the detection limits 9.0 nM, 9.0 nM and 0.08 μM, respectively. The CuO-rGR/1M3OIDTFB/CPE displayed high performance for the determination of CL, AA and UA in real samples.

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Topics: Ascorbic acid (57%), Carbon paste electrode (54%), Buffer solution (53%)

230 Citations


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