A Novel Fluorometric Bio-Sensing-Based Arsenic Detection System for Groundwater
01 Sep 2017-IEEE Sensors Journal (Institute of Electrical and Electronics Engineers (IEEE))-Vol. 17, Iss: 17, pp 5391-5398
TL;DR: In this paper, a low cost, portable, and eco-friendly field test system is required to monitor the levels of arsenic in drinking water procured from different tube wells and open wells.
Abstract: Arsenic that adversely affects human health is frequently reported in certain regions in India and other parts of the world. A low cost, portable, and eco-friendly field test system is required to monitor the levels of arsenic in drinking water procured from different tube wells and open wells. This paper presents the construction of biosensor strain by transforming E. coliDH $5\alpha $ with the plasmid pJSKV51 carrying in-frame promoter operator region as well as entire arsR gene, part of arsD gene and enhanced green fluorescent protein (EGFP) gene. This strain was able to induce EGFP in presence of arsenic III ion. This paper further presents a novel EGFP-based bio-electronic sensing methodology to develop a low-cost battery operated system to measure the fluorescence emitted by biosensor strain and display the numerical values of the concentrations of arsenic in water samples. In this method, a blue light of 480 nm excites the EGFP to produce a green fluorescence signal in proportion to arsenic concentration. The developed device includes a compact embedded system to measure the signal fluorescence using light sensor, programmable controllers and liquid crystal display to show the corresponding concentration of the arsenic in ppb. The experimental results show that the system can detect and display over a range from 5 to 100 ppb.
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TL;DR: In this paper , the authors comprehensively overviewed the electrochemical sensors that employ nanomaterials (nanoparticles/colloids or quantum dots, carbon dots, or nanoscaled metal-organic frameworks, etc.) to detect a variety of biological macromolecules based on fluorescent emission spectra.
45 citations
TL;DR: The central agenda of this paper is to develop an understanding into the nano-enabled methods for arsenic detection with an emphasis on strategic fabrication of nanostructures and the modulation of nanomaterial chemistry in order to strengthen the knowledge into novel nano- enabled solutions for arsenic contamination.
39 citations
TL;DR: In this paper, a bioPAD was used to detect doxycycline concentrations in both human urine and raw bovine serum, using fluorescence as read-out.
Abstract: To combat pharmaceutical counterfeiting and antibiotic resistance in low- and middle-income countries (LMIC), there is a need for improved low-cost, portable methods that monitor pharmaceutical concentrations relevant to dosage forms and physiological fluids. To address this problem, we turned to paper analytical devices (PADs), and have recently extended this technology by incorporating whole cell yeast biosensors into PADs to create biological paper analytical devices (bioPADs). The goals of the work presented here were to build on our initial bioPAD technology by showing that fluorescence can be used as a read-out and that the yeast-based system can function in complex matrices. More specifically, we embedded the bioengineered yeast on a paper test strip and showed that this simple biosensor can detect physiologically relevant concentrations of doxycycline in both human urine and raw bovine serum. In this work we also manufactured an inexpensive and portable device capable of reading the fluorescent signal of the bioPAD. This work demonstrates the untapped potential of fluorescent yeast biosensors for use in LMICs.
14 citations
TL;DR: A genetically encoded biosensor in vitro with an arsenical resistance operon repressor (ArsR) and GFP reporter gene and a standard procedure for arsenic detection in vitro is established to facilitate the practical application of genetically coded biosensors in TP monitoring.
8 citations
01 Nov 2018
TL;DR: In this article, a simple theory was described that will detect the arsenic percentage of water easily and a modern arsenic detecting kit and simple color sensor have been used for developing the system, some sample of water was collected from different region of Khulna, Bangladesh to measure the percentage of arsenic contamination.
Abstract: Arsenic is one kind of chemical element that can poison surface water with causing many human difficulties. For detecting and giving a clear view of arsenic contamination in water there are different types of researches going on. Most of the researches are developed for laboratory work. In this work, not only a system was developed but also a simple theory was described that will detect the arsenic percentage of water easily. Modern arsenic detecting kit and simple color sensor have been used for developing the system. Some sample of water was collected from different region of Khulna, Bangladesh to measure the percentage of arsenic contamination. The data was recorded and analyzed to verify the system.
6 citations
Cites background from "A Novel Fluorometric Bio-Sensing-Ba..."
...Most challenge now-a-days is the process simplicity of the detection with a compact device that will come in low cost[13]....
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References
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TL;DR: The paper provides an overview of the state-of-the-art knowledge on the alternative As free drinking water and various technologies (oxidation, coagulation flocculation, adsorption, and microbial) for mitigation of the problem of As contamination of groundwater.
Abstract: Arsenic contamination of groundwater in different parts of the world is an outcome of natural and/or anthropogenic sources, leading to adverse effects on human health and ecosystem. Millions of people from different countries are heavily dependent on groundwater containing elevated level of As for drinking purposes. As contamination of groundwater, poses a serious risk to human health. Excessive and prolonged exposure of inorganic As with drinking water is causing arsenicosis, a deteriorating and disabling disease characterized by skin lesions and pigmentation of the skin, patches on palm of the hands and soles of the feet. Arsenic poisoning culminates into potentially fatal diseases like skin and internal cancers. This paper reviews sources, speciation, and mobility of As and global overview of groundwater As contamination. The paper also critically reviews the As led human health risks, its uptake, metabolism, and toxicity mechanisms. The paper provides an overview of the state-of-the-art knowledge on the alternative As free drinking water and various technologies (oxidation, coagulation flocculation, adsorption, and microbial) for mitigation of the problem of As contamination of groundwater.
414 citations
"A Novel Fluorometric Bio-Sensing-Ba..." refers background in this paper
...Arsenic has been linked to cancer of the bladder, lungs, skin, kidney, nasal passages, liver, and prostate [9]....
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TL;DR: Renaturation of Aequorea green-fluorescent protein (A-GFP) was achieved for the first time following denaturation in guanidine-HCl or acid.
313 citations
"A Novel Fluorometric Bio-Sensing-Ba..." refers result in this paper
...These variations in flourescence agree with the reported results for wild type GFP [29]....
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TL;DR: The development of a set of bacterial biosensors based on a nonpathogenic laboratory strain of Escherichia coli, the natural resistance mechanism of E. coli against arsenite and arsenate, and three reporter proteins: bacterial luciferase, beta-galactosidase and Green Fluorescent Protein (GFP) is described.
Abstract: Testing for arsenic pollution is commonly performed with chemical test kits of unsatisfying accuracy. Bacterial biosensors are an interesting alternative as they are easily produced, simple, and highly accurate devices. Here, we describe the development of a set of bacterial biosensors based on a nonpathogenic laboratory strain of Escherichia coli, the natural resistance mechanism of E. coli against arsenite and arsenate, and three reporter proteins: bacterial luciferase, beta-galactosidase and Green Fluorescent Protein (GFP). The biosensors were genetically optimized to reduce background expression in the absence of arsenic. In calibration experiments with the biosensors and arsenite-amended potable water, arsenite concentrations at 4 microg of As/L (0.05 microM) were routinely and accurately measured. The currently most quantitative system expressed the bacterial luciferase as reporter protein, responding proportional with a concentration range between 8 and 80 microg of As/L. Sensor cells could be stored as frozen batches, resuspended in plain media, and exposed to the aqueous test sample, and light emission was measured after 30-min incubation. Field testing for arsenite was achieved with a system that contained beta-galactosidase, producing a visible blue color at arsenite concentrations above 8 microg/L. For this sensor, a protocol was developed in which the sensor cells were dried on a paper strip and placed in the aqueous test solution for 30 min after which time color development was allowed to take place. The GFP sensor showed good potential for continuous rather than end point measurements. In all cases, growth of the biosensors and production of the strip test was achieved by very simple means with common growth media, and quality control of the sensors was performed by isolating the respective plasmids with the genetic constructs according to simple standard genetic technologies. Therefore, the biosensor cells and protocols may offer a realistic alternative for measuring arsenic contamination in potable water.
307 citations
"A Novel Fluorometric Bio-Sensing-Ba..." refers methods in this paper
...Luminescent-based biosensor [16], [17], fluorescence-based biosensor [18], chromogenic systems based on X-gal [19] and pH-based biosensor [20] have been used for the detection of As....
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TL;DR: In this paper, the terms recovery and apparent recovery are recommended to avoid confusion caused by the use of the term recovery to cover two distinct situations: the yield of a preconcentration or extraction stage of an analytical process (where recovery is recommended) and the quantity observed value/reference value, obtained using an analytical procedure that involves a calibration graph.
Abstract: The terms recovery and apparent recovery are recommended to avoid confusion caused by the use of the term recovery to cover two distinct situations. These situations deal with: (a) the yield of a preconcentration or extraction stage of an analytical process (where recovery is recommended) and (b) the quantity observed value/reference value, obtained using an analytical procedure that involves a calibration graph (where apparent recovery is recommended).
297 citations
TL;DR: Sb is relatively unreactive and immobile in the surface layers of the soil, remaining where it is deposited rather than leaching into lower horizons and contaminating ground water, indicating that soil contamination by Sb is not a severe risk to the environment or human health.
196 citations
"A Novel Fluorometric Bio-Sensing-Ba..." refers background in this paper
...[25] described the measurement of bio available arsenic in soil samples utilizing V....
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