Figure 4. Transfection efficiency in BATkl2 cells: siRNA and plasmid-mediated expression. (A) Prkaca mRNA expression of BATkl2 cells 2 days after transfection with the indicated siRNA in the undifferentiated state (n=3 cultures). si NC: non-specific siRNA control. (B) Prkaca mRNA expression of BATkl2 cells at day 8 of differentiation after transfection with the indicated siRNA at 5 days of differentiation (n=3 cultures). (C,D) Merged phase-contrast and fluorescence microscopy images of BATkl2 cells 24 hours after transfection with pEGFP-N1 plasmid in the undifferentiated state. (C) non-transfected cultures. Data represent mean and standard deviation.
...read more