A Perspective on Enzyme Catalysis
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...), and they have a minimal effect on the overall rates of biological processe...
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...It also implies that the chemical shifts between the two states (Dq),and hence their structural differences, are identical for wild-type CypA and all CypA mutants (scheme (3)); Supplementary Table S2)....
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...It is also noteworthy that the calculated chemical-shift differences (equation (1)) between Emajor and Eminor (qEmajor 2 qEminor; scheme (3)) are similar to those between EScis and EStrans (qEScis 2 qEStrans; scheme (2)) for residues remote from the substrate, suggesting that the cis substrate binds Emajor to form EScis, whereas the trans substrate binds Eminor to form EStrans....
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