A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.
About: This article is published in Journal of Lipid Research.The article was published on 1964-04-01 and is currently open access. It has received 369 citations till now. The article focuses on the topics: Thin-layer chromatography & Reagent.
Citations
More filters
TL;DR: A rapid method is described for charring 5-300 micrograms of lipids (with concentrated sulfuric acid in a test tube) and estimating them with a reproducibility of +/-1%.
1,037 citations
TL;DR: The various functions storage lipids serve during the life history stages of zooplankton are very complex and still not fully understood and hence offer a multitude of fascinating research perspectives.
Abstract: Zooplankton storage lipids play an important role during reproduction, food scarcity, ontogeny and diapause, as shown by studies in various oceanic regions. While triacylglycerols, the primary storage lipid of terrestrial animals, are found in almost all zooplankton species, wax esters are the dominant storage lipid in many deep-living and polar zooplankton taxa. Phospholipids and diacylglycerol ethers are the unique storage lipids used by polar euphausiids and pteropods, respec- tively. In zooplankton with large stores of wax esters, triacylglycerols are more rapidly turned over and used for short-term energy needs, while wax esters serve as long-term energy deposits. Zooplankton groups found in polar, westerlies, upwelling and coastal biomes are characterized by accumulation of large lipid stores. In contrast, zooplankton from the trades/tropical biomes is mainly composed of omnivorous species with only small lipid reserves. Diapausing copepods, which enter deep water after feeding on phytoplankton during spring/summer blooms or at the end of upwelling periods, are characterized by large oil sacs filled with wax esters. The thermal expansion and com- pressibility of wax esters may allow diapausing copepods and other deep-water zooplankton to be neutrally buoyant in cold deep waters, and they can thus avoid spending energy to remain at these depths. Lipid droplets are often noted in zooplankton ovaries, and a portion of these droplets can be transferred to developing oocytes. In addition to lipid droplets, zooplankton eggs have yolks with lipovitellin, a lipoprotein with approximately equal amounts of protein and lipid. The lipovitellin lipid is predominantly phosphatidylcholine, so during reproduction females must convert a portion of their storage lipid into this phospholipid. Developing embryos use their lipovitellin and lipid droplets for energy and materials until feeding begins. The various functions storage lipids serve during the dif- ferent life history stages of zooplankton are very complex and still not fully understood and hence offer a multitude of fascinating research perspectives.
665 citations
TL;DR: A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids.
547 citations
544 citations
TL;DR: Linolenic acid was converted to 20:5 omega 3 and 22:6 omega 3 intensively in the rainbow trout, moderately in the ayu, eel and prawn, but slightly in the red sea bream, rockfish and globefish.
Abstract: 1. 1. [1-14C]linolenic acid was injected into the rainbow trout, Salmo gairdnerii, ayu, Plecoglossus altivelis, eel, Anguilla japonica, red sea bream, Chrysophrys major, rockfish, Sebastiscus marmoratus, globefish, Fugu rubripes rubripes and prawn, Penaeus japonicus (molting stage D1″-D2), and the bioconversion of linolenic acid (18:3ω3) to highly unsaturated fatty acids such as eicosapentaenoic (20:5ω3) and docosahexaenoic (22:6ω3) acids was investigated. 2. 2. Linolenic acid was converted to 20:5ω3 and 22:6ω3 intensively in the rainbow trout, moderately in the ayu, eel and prawn, but slightly in the red sea bream, rockfish and globefish. 3. 3. These results were discussed in relation to the essential fatty acid requirements of the aquatic animals.
405 citations
References
More filters
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
59,550 citations
TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
454 citations
TL;DR: In this article, a method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determination via their hydroxamic acids has been developed, which is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty acids.
Abstract: A method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determinationvia their hydroxamic acids has been developed. Esters of different types are separated on silica gel plates, the spots or zones are scraped from the plates, the esters are extracted from the silica gel, and the iron hydroxamic acid complexes are formed. The latter are then measured by colorimetry. The method is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty esters as well as for mixtures of mono-, di-, and triglycerides. Used in conjunction with gas chromatography this method permits the fatty acid composition of seed oils containing oxygenated fatty acids to be measured in about 3 hours. The amount required for the total analysis is 1 to 10 mg. per determination, depending upon composition.
122 citations
96 citations
TL;DR: The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.
Abstract: SummaryThe use of thin layer chromatography for analyzing phosphatides and their hydrolysis products quantitatively is described and applied to the analysis of serum phosphatides. The results are similar to those obtained with silicic acid impregnated paper chromatography and column techniques, though the time for the analysis is markedly shortened.
63 citations