Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
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The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.Abstract:
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can...read more
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Journal ArticleDOI
Influence of diet on the distribution of nitrogen isotopes in animals
Michael J. DeNiro,Samuel Epstein +1 more
TL;DR: In this article, the influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition and found that the variability of the relationship between the δ(15)N values of animals and their diets is greater for different individuals raised on the same diet than for the same species raised on different diets.
Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals
Michael J. DeNiro,Samuel Epstein +1 more
TL;DR: In this article, the influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition and found that the variability of the relationship between the δ^(15)N values of animals and their diets is greater for different individuals raised on the same diet than for the same species raised on different diets.
Journal ArticleDOI
An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids
David E. Minnikin,Anthony G. O'Donnell,Michael Goodfellow,G. Alderson,M. Athalye,A. Schaal,J. H. Parlett +6 more
TL;DR: A simple small-scale procedure for the sequential extraction of isoprenoid quinones and polar lipids from bacterial cells was developed and the extracted organisms were analyzed to give a polar lipid extract.
Journal ArticleDOI
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters
TL;DR: Kinetic analysis indicates that TPA can substitute for diacylglycerol and greatly increases the affinity of the enzyme for Ca2+ as well as for phospholipid, and various phorbol derivatives which have been shown to be active in tumor promotion are also capable of activating this protein kinase in in vitro systems.
Book ChapterDOI
4 Lipid and Cell-Wall Analysis in Bacterial Systematics
Kazuo Komagata,Ken-ichiro Suzuki +1 more
TL;DR: Progress in the biochemistry of microorganisms have revealed that cell-component analysis can be effectively applied to bacterial systematics, providing the basis of chemotaxonomy, and DNA homology data contribute to the clarification, not only of the relatedness of two organisms, but also of the evaluation of phenotypic characteristics.
References
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Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
Journal ArticleDOI
Storage of Frozen Plaice Fillets
W. J. Dyer,Margaret L. Morton +1 more
TL;DR: Taste panel assessments of taste, texture, and grade, and estimations of soluble actomyosin and of fat spoilage on plaice fillets after frozen storage at + 10°F are reported.
Journal ArticleDOI
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Journal ArticleDOI
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
TL;DR: A rapid, precise analytical method of fat determination based on observations of the variation of the efficiency of acetone as an extractant of fats from biological materials has been developed.