Journal ArticleDOI
A reinvestigation of inhibitors of glyoxalase I
Subhankar Ray,Ray Mk +1 more
Reads0
Chats0
TLDR
It is found that nucleotides such as ATP, GTP and different classes of other reagents based on transition state analogy (D-isoascorbate, dihydroxyfumaric acid, rhodizonic acid) do not inhibit yeast or goat liver glyoxalase I.Abstract:
It has been reported earlier that nucleotides, nucleosides and a series of structurally related compounds as well as compounds based on transition state analogy inhibit yeast glyoxalase I. In our study on the metabolic regulation of glyoxalase I, we have found that nucleotides such as ATP, GTP and different classes of other reagents based on transition state analogy (D-isoascorbate, dihydroxyfumaric acid, rhodizonic acid) do not inhibit yeast or goat liver glyoxalase I. The reported inhibition of glyoxalase I by these compounds has been found to be due to the interference of these compounds with the absorbancy at 240 nm of S-D-lactoylglutathione formed by the glyoxalase I reaction.read more
Citations
More filters
Journal ArticleDOI
Investigation on glyoxalase I inhibitors.
John F. Barnard,John F. Honek +1 more
TL;DR: Examination of some of the aforementioned compounds has revealed that squaric acid does not function as an inhibitor of glyoxalase I and several other compounds are much less effective in this regard than previously reported.
Journal ArticleDOI
Molecular docking and dynamic studies of a potential therapeutic target inhibiting glyoxalase system: Metabolic action of the 3, 3 '- [3- (5-chloro-2-hydroxyphenyl) -3-oxopropane-1, 1-diyl] - Bis-4-hydroxycoumarin leads overexpression of the intracellular level of methylglyoxal and induction of a pro-apoptotic phenomenon in a hepatocellular carcinoma model
Nadia Taibi,Qosay Al-Balas,Nadjia Bekari,Oualid Talhi,Ghazi A. Al Jabal,Yasmine Benali,Rachid Ameraoui,Mohamed Hadjadj,Amina Taïbi,Zahra Mouna Boutaiba,Mohamed Abou-mustapha,Farida Khammar,Fayçal Dergal,Ridha Hassaine,Leila Boukenna,Khaldoun Bachari,Artur M. S. Silva +16 more
TL;DR: In this article, the authors demonstrate the inhibitory effect of 3, 3'-[3-(5-chloro-2-hydroxyphenyl)-3-oxopropane-1, 1-diyl] Bis (4-hydroxycoumarin) on the glyoxalase system, and indirectly its anticancer activity.
Journal ArticleDOI
Interaction of aldehydes with glyoxalase I and the status of several aldehyde metabolizing enzymes of Ehrlich ascites carcinoma cells.
TL;DR: The results indicate that D, and L-lactaldehyde are strong non-competitive inhibitors of glyoxalase I and the effect with the D-isomer is more pronounced, whereas both D,L-glyceraldehyde and acetaldehyde are moderately inhibitory and the nature of inhibition is strictly competitive.
References
More filters
Journal ArticleDOI
Isolation of methylglyoxal synthase from goat liver.
Subhankar Ray,Manju Ray +1 more
TL;DR: An enzyme fraction which specifically catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate has been isolated and partially purified from goat liver and appears to be substantially free from glyoxalase I, reduced glutathione, and triosephosphate isomerase.
Journal ArticleDOI
The enzymatic oxidation of 1,2-propanediol phosphate to acetol phosphate.
TL;DR: The product of the oxidation of propanediol-P by rabbit muscle oc-glycerol phosphate dehydrogenase has been established as acetol-P, CH3COCH20P03H2, and attempts to demonstrate this reaction in rat liver homogenates have been unsuccessful.
Journal ArticleDOI
Aminoacetone oxidase from goat liver. Formation of methylglyoxal from aminoacetone.
Manju Ray,Subhankar Ray +1 more
TL;DR: An enzyme which oxidizes aminoacetone to methylglyoxal has been purified from the particulate fraction of goat liver and is highly sensitive to carbonyl group reagents.
Journal ArticleDOI
Characterization of methylglyoxal synthase in Saccharomyces cerevisiae
Kousaku Murata,Yasuki Fukuda,Kunihiko Watanabe,Toshihiko Saikusa,Makoto Shimosaka,Akira Kimura +5 more
TL;DR: Methylglyoxal synthase in Saccharomyces cerevisiae was purified approximately 300 folds from cell extracts with 20% of activity yield and during purification procedures, polymorphic behaviours of the enzyme were observed.
Journal ArticleDOI
The structure of a glyoxalase I inhibitor and its chemical reactivity with SH-compounds.
Hideo Chimura,Hikaru Nakamura,Tomohisa Takita,Tomio Takeuchi,Hamao Umezawa,Kuniki Kato,Seiichi Saito,Takayuki Tomisawa,Yoichi Iitaka +8 more
TL;DR: The structure of a glyoxalase I inhibitor (I), isolated from a cultured broth of Streptomyces griseosporeus, was found to be 2-crotonyloxymethyl-4,5,6-trihydroxy-cyclohex-2-enone by chemical studies.