Journal ArticleDOI
A self-luminous bifunctional bacteria directed fluorescent immunosensor for the simultaneous detection and quantification of three pathogens in milk
Reads0
Chats0
TLDR
A novel multiplex fluorescent competitive immunosensor using self-luminous bacteria as a bifunctional probe for the simultaneous detection and quantification of multiple pathogens and provides an alternative multiplexing analytical platform as a powerful and rapid diagnostic tool for monitoring foodborne pathogen contamination.Abstract:
Herein, we report a novel multiplex fluorescent competitive immunosensor using self-luminous bacteria as a bifunctional probe for the simultaneous detection and quantification of multiple pathogens. The self-luminous bacteria are prepared through the facile one-step co-culture of bacteria and organic fluorescent dyes. Cronobacter muytjensii, Listeria monocytogenes, and Escherichia coli O157:H7 are fluorescently labelled with 7-hydroxycoumarin, fluorescein isothiocyanate, and rhodamine B, respectively, due to the dyes small spectral overlap in excitation/emission. The obtained fluorescent bacteria retain specific immunorecognition activity, but exhibit excellent fluorescent sensing ability. By using these fluorescent bacteria as bifunctional probes for competitive recognition and optical sensing, we achieved the sensitive and simultaneous quantitative determination of three pathogens with sensitivities of below 8.6 × 103 CFU/mL. The sensitivities can be significantly enhanced to 1 CFU/mL in real milk by integrating with a pre-incubation step, showing great potential for the ultrasensitive determination of trace bacteria and even single colony with the overall detection time of less than 10 h. In summary, this work provides an alternative multiplexing analytical platform as a powerful and rapid diagnostic tool for monitoring foodborne pathogen contamination.read more
Citations
More filters
Journal ArticleDOI
Enzyme-free catalytic hairpin assembly reaction-mediated micro-orifice resistance assay for the ultrasensitive and low-cost detection of Listeria monocytogenes
TL;DR: In this paper , a micro-orifice resistance technique combined with the aggregation of polystyrene (PS) microspheres constructed by the catalytic hairpin assembly reaction (CHA) was used to detect L. monocytogenes.
Journal ArticleDOI
Enzyme-free catalytic hairpin assembly reaction-mediated micro-orifice resistance assay for the ultrasensitive and low-cost detection of Listeria monocytogenes.
Li Ren,Feng Hong,Yiping Chen +2 more
TL;DR: A novel, enzyme-free, dual-signal amplification approach to detect L. monocytogenes based on the micro-orifice resistance technique combined with the aggregation of polystyrene microspheres constructed by the catalytic hairpin assembly reaction (CHA).
Journal ArticleDOI
Advantages of aggregation-induced luminescence microspheres compared with fluorescent microspheres in immunochromatography assay with sandwich format.
Xiaxia Wei,Xirui Chen,Zilong Liu,Ge Xu,Guoxin Chen,Xiangkai Lin,Wenchao Zhan,Xiaolin Huang,Yonghua Xiong +8 more
TL;DR: In this paper , two green emitted fluorescent microspheres (AIEFMs) and fluorescein isothiocyanate FMs (FITCFMs) were used as the labeling markers of ICA for procalcitonin (PCT) detection with the sandwich format.
Journal ArticleDOI
Fluorescent/electrochemical dual-signal response biosensing strategy mediated by DNAzyme-ferrocene-triggered click chemistry for simultaneous rapid screening and quantitative detection of Vibrio parahaemolyticus
Journal ArticleDOI
Multiplex Detection of Infectious Diseases on Microfluidic Platforms
TL;DR: In this paper , the authors present the recent advances in microfluidic platforms used for multiplex detection of infectious diseases, including microfluide immunosensors and microfluidity nucleic acid sensors, and discuss the current challenges, commercialization, and prospects.
References
More filters
Journal ArticleDOI
Visualization of RNA-Quadruplexes in Live Cells
Aurélien Laguerre,Kyle R. Hukezalie,Pascale Winckler,Fares Katranji,Gaëtan Chanteloup,Marc Pirrotta,Jean-Marie Perrier-Cornet,Judy M. Y. Wong,David Monchaud +8 more
TL;DR: N-TASQ is reported on, a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe that enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexe in live cells.
Journal ArticleDOI
An Improved Ultrasensitive Enzyme-Linked Immunosorbent Assay Using Hydrangea-Like Antibody–Enzyme–Inorganic Three-in-One Nanocomposites
TL;DR: This work first coimmobilized the enzyme, antibody, and Cu3(PO4)2 into a three-in-one hybrid protein-inorganic nanoflower to enable it to possess dual functions: (1) the antibody portion retains the ability to specifically capture the corresponding antigen; (2) the nan oflower has enhanced enzymatic activity and stability to produce an amplified signal.
Journal ArticleDOI
Biosensors based on modularly designed synthetic peptides for recognition, detection and live/dead differentiation of pathogenic bacteria.
Xiaobo Liu,Mouna Marrakchi,Mouna Marrakchi,Mouna Marrakchi,Dawei Xu,He Dong,Silvana Andreescu +6 more
TL;DR: The proposed methodology provides a universal platform for the detection of bacterial pathogens based on engineered peptides, as alternative to the most commonly used immunological and gene based assays.
Journal ArticleDOI
A Meta-Analysis of Major Foodborne Pathogens in Chinese Food Commodities Between 2006 and 2016.
TL;DR: Presence of major food pathogens in Chinese foods in decreasing order of prevalence equates the risk of microbiological food safety in China with other developed countries rather than the developing countries, which justifies the need of novel perspectives for formulating policies on microbiologicalFood safety and risk mitigation.
Journal ArticleDOI
Fluorescent Immunoassay for the Detection of Pathogenic Bacteria at the Single-Cell Level Using Carbon Dots-Encapsulated Breakable Organosilica Nanocapsule as Labels
TL;DR: Carbon dots-encapsulated breakable organosilica nanocapsules were facilely prepared and used as advanced fluorescent labels for ultrasensitive detection of Staphylococcus aureus and were amplified by 2 orders of magnitude.
Related Papers (5)
Fabricating Upconversion Fluorescent Probes for Rapidly Sensing Foodborne Pathogens
Fluorescent nanoparticles for multiplexed bacteria monitoring.
Ultrasensitive peptide-based multiplexed electrochemical biosensor for the simultaneous detection of Listeria monocytogenes and Staphylococcus aureus
Shimaa Eissa,Mohammed Zourob +1 more