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Journal ArticleDOI

A simple approach to the toad's ( Bufo melanostictus ) nerver muscle preparation

15 May 1976-Cellular and Molecular Life Sciences (Experientia)-Vol. 32, Iss: 5, pp 589-590
TL;DR: A simple and modified dorsal approach method has been made in the toad's (Bufo melanostictus) sciatic gastrocnemius nerve-musle preparation that incurs less blood loss, time consumption, nerve damage and visceral spoil compared to conventional ventral approach method.
Abstract: A simple and modified dorsal approach method has been made in the toad's (Bufo melanostictus) sciatic gastrocnemius nerve-muscle preparation. This method incurs less blood loss, time consumption, nerve damage and visceral spoil compared to conventional ventral approach method.
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Journal ArticleDOI
TL;DR: In hibernating snailsHelix lucorum, the effect of 5-HT on neurons of an identified group is mainly hyperpolarization associated with a conductance increase, while, in active snails, theEffect is depolarization and a decrese of the membrane conductance.
Abstract: In hibernating snails Helix lucorum, the effect of 5-HT on neurons of an identified group is mainly hyperpolarization associated with a conzation conductance increase, while, inactive snails, the effect is de polarization decrease of the membrane conductance.

7 citations

Journal Article
TL;DR: The therapeutic effect of this concoction might be due in part to the presence of tobacco or similar constituent in the cow urine concoction, like muscular weakness and hyper-excitability of the nerve-skeletal system, seen in children convulsion.
Abstract: Cow urine concoction (CUC) is a local herbal preparation administered to children having convulsion in southern part of Nigeria. We investigated the effect of full preparation (FP) and modified preparation (MP) [without tobacco] of CUC and nicotine on the nerve-muscle preparation in common African toad, Bufo regularis. The result showed that nicotine significantly potentiated the contraction of skeletal muscle at lower doses, and at a higher dose the sensitivity of neuro-muscular junction was significantly reduced (desensitization). At lower doses of FP of CUC, the contraction of skeletal muscle was slightly increased than the nicotine but was not significant but the response was very similar to that obtained at higher doses. The finding indicates that nicotine and FP of CUC have something in common in stimulating muscle contraction. MP of CUC did not show any significant response at graded doses. It was concluded from the results that the therapeutic effect of this concoction might be due in part to the presence of tobacco or similar constituent in the cow urine concoction. The effect of „FP” of CUC in this study can be employed clinically in some disorders of the nerve-skeletal muscle system, like muscular weakness and hyper-excitability of the nerve-skeletal system, seen in children convulsion.

4 citations


Cites methods from "A simple approach to the toad's ( B..."

  • ...The objective of this method is to minimize blood loss, abdominal visceral is not disturbed and an intestinal content does not come out to spoil the nerve and the nerve is least damaged [9]....

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  • ...The method described by Dasgupta and Muherjee method [9] was used for nerve-muscle preparation is briefly de-scribed as follows: The toad brain was pithed with a blunt seeker at a point of depression, the toad was laid on the dissecting board with dorsal surface uppermost, the urostyle was removed then the sciatic nerve on either side was exposed....

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