A simple method for the isolation and purification of total lipides from animal tissues.
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In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.About:
This article is published in Journal of Biological Chemistry.The article was published on 1957-05-01 and is currently open access. It has received 59550 citations till now. The article focuses on the topics: Triglyceride transport.read more
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Microdetermination of long-chain fatty acids in plasma and tissues.
Vincent P. Dole,Hans Meinertz +1 more
TL;DR: The present paper outlines the phase relations of the ternary system at room temperature, gives partition coefficients of fatty acids and other materials in a standard two-phase extraction mixture, discusses use of the system in countercurrent estraction, and outlines some modifications of the original procedure that have developed during five years of analytical work in this laboratory.
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Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes)
TL;DR: The lipid composition of the vesicles shows the high sphingomyelin content characteristic of sheep red cell plasma membranes, but not white cell or platelet membranes, consistent with the conclusion that the vESicles are of reticulocyte origin.
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Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses
David M. Post,Craig A. Layman,D. Albrey Arrington,Gaku Takimoto,John Quattrochi,Carman G Montaña +5 more
TL;DR: The results indicate that lipid extraction or normalization is most important when lipid content is variable among consumers of interest or between consumers and end members, and when differences in δ13C between end members is <10–12‰.
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A simple, specific spray for the detection of phospholipids on thin-layer chromatograms.
John C. Dittmer,Robert L. Lester +1 more
TL;DR: A chemical reagent spray specific for phosphate esters, based on molybdenum blue, gives an instantaneous, specific reaction with phospholipids on silica gel or alumina thin layer chromatography (TLC) plates.
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GPR120 is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-Inflammatory and Insulin Sensitizing Effects
Da Young Oh,Saswata Talukdar,Eun Ju Bae,Takeshi Imamura,Hidetaka Morinaga,WuQiang Fan,Pingping Li,Wendell J. Lu,Steven M. Watkins,Jerrold M. Olefsky +9 more
TL;DR: GPR120 is a functional omega-3 FA receptor/sensor and mediates potent insulin sensitizing and antidiabetic effects in vivo by repressing macrophage-induced tissue inflammation.
References
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Preparation of lipide extracts from brain tissue.
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
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Isolation of brain strandin, a new type of large molecule tissue component.
Jordi Folch,S. Arsove,J.A. Meath +2 more
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
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