A simple method for the quantitative analysis of phospholipids separated by thin layer chromatography
TL;DR: A rapid, simple and reproducible method for the quantitative determination of phospholipids after separation by thin layer chromatography has been described and the method was compared with the generally cited methods used for the same purpose.
About: This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 154 citations till now. The article focuses on the topics: Thin-layer chromatography.
Citations
More filters
TL;DR: A simple procedure for preparing a stable stock reagent an working reagents for the detection and determination of phospholipids is proposed, and a simple, rapid and accurate thin-layer chromatographic technique is suggested.
568 citations
TL;DR: The findings with the synthetic peptide suggest that a direct interaction between the Ca2(+)-ATPase and the hydrophilic portion of phospholamban may be one of the mechanisms for regulation.
155 citations
TL;DR: Evidence is provided that acid sphingomyelinase activity plays an essential role in the regulation of glucose metabolism by regulating the hepatic accumulation of triacylglycerides and sphingolipids during consumption of a diet rich in saturated fats.
85 citations
Journal Article•
TL;DR: In this article, cow milk was fortified with calcium at the rate of 50mg/100ml using three salts of calcium, viz. calcium chloride, calcium lactate and calcium gluconate.
Abstract: The objective of the study was to fortify calcium in cow milk in order to prepare calcium-enriched heat-stable milk for individuals who may not ingest enough calcium to meet minimum daily requirements. Therefore, cow milk was fortified with calcium at the rate of 50 mg/100 ml using three salts of calcium, viz. calcium chloride, calcium lactate and calcium gluconate. Upon addition of calcium salts, there was a marked drop in the pH and heat stability. However, restoration of pH to the original value with the addition of disodium phosphate stabilized the fortified milk and enhanced its heat stability over unfortified milk. The maximum in heat stability (HCT) of calcium-fortified cow milk samples remained slightly higher than that of unfortified milk. Metabolic study on mice revealed that calcium bioavailability of cow milk fortified with calcium lactate and calcium gluconate and stabilized with disodium phosphate was slightly higher than unfortified cow milk. Fortification of cow milk with calcium and restoration of its pH resulted in a calcium to phosphorus ratio still greater than one, which is considered ideal for retention of calcium in the body.
82 citations
TL;DR: In this paper, cow milk was fortified with calcium at the rate of 50mg/100ml using three salts of calcium, viz. calcium chloride, calcium lactate and calcium gluconate.
Abstract: The objective of the study was to fortify calcium in cow milk in order to prepare calcium-enriched heat-stable milk for individuals who may not ingest enough calcium to meet minimum daily requirements. Therefore, cow milk was fortified with calcium at the rate of 50 mg/100 ml using three salts of calcium, viz. calcium chloride, calcium lactate and calcium gluconate. Upon addition of calcium salts, there was a marked drop in the pH and heat stability. However, restoration of pH to the original value with the addition of disodium phosphate stabilized the fortified milk and enhanced its heat stability over unfortified milk. The maximum in heat stability (HCT) of calcium-fortified cow milk samples remained slightly higher than that of unfortified milk. Metabolic study on mice revealed that calcium bioavailability of cow milk fortified with calcium lactate and calcium gluconate and stabilized with disodium phosphate was slightly higher than unfortified cow milk. Fortification of cow milk with calcium and restoration of its pH resulted in a calcium to phosphorus ratio still greater than one, which is considered ideal for retention of calcium in the body.
81 citations
References
More filters
1,329 citations
884 citations
TL;DR: Bandurski, R. S., Wilson, L. G. & Squires, G. S. (1956).
Abstract: Bandurski, R. S., Wilson, L. G. & Squires, G. L. (1956). J. Amer. chem. Soc. 78, 6408. Caputto, R., Leloir, L. F., Cardini, C. E. & Paladini, A. C. (1950). J. biol. Chem. 184, 333. Cohen, S. S. & Scott D. B. McNair (1950). Science, 111, 543. Cremer, H. D. & Tiselius, A. (1950). Biochem. Z. 320, 273. D'Abramo, F. &A Lipmann, F. (1957). Biochim. biophys. Acta, 25, 211. DeMeio, R. H. & Tkacz, L. (1950). Arch. Biochem. 27, 242. Gregory, J. D. & Nose, Y. (1957). Fed. Proc. 16, 189. Hanes, C. S. & Isherwood, F. A. (1949). Nature, Lond., 164, 1107. Hilz, H. & Lipmann, F. (1955). Proc. nat. Acad. Sci., Wash., 41, 880. Klemperer, H. (1955). D.Phil. Thesis: University of Oxford. Layton, L. L. & Frankel, D. R. (1951). Arch. Biochem. Biophy8. 31, 161. Loewi, G. & Kent, P. W. (1957). Biochem. J. 65, 550. Markham, R. & Smith, J. D. (1949). Biochem. J. 45, 294. Meyer, K. & Schwartz, D. E. (1950). Helv. chim. Acta, 33, 1651. Paladini, A. C. & Leloir, L. F. (1952). Biochem. J. 51, 426. Partridge, S. M. (1946). Nature, Lond., 158, 270. Partridge, S. M. (1949). Nature, Lond., i164, 443. Pasternak, C. A. & Kent, P. W. (1958). Biochem. J. 68, 452. Pasternak, C. A., Kent, P. W. & Davies, R. E. (1958). Biochem. J. 68, 212. Robbins, P. W. & Lipmann, F. (1956a). J. Amer. chem. Soc. 78, 2652. Robbins, P. W. & Lipmann, F. (1956b). J. Amer. chem. Soc. 78, 6409. Suzuki, S., Takahashi, N. & Egami, F. (1957). Biochim. biophy8. Acta, 24, 444. Werner, I. (1953). Upp8ala Ldkf6ren, F6rh. 58, 1. Williams, R. T. (1947). Detoxication Mechani8ms, p. 70. London: Chapman and Hall. Wilson, L. G. & Bandurski, R. S. (1956). Arch. Biochem. Biophys. 62, 503. Wyatt, G. R. (1951). Biochem. J. 48, 584.
492 citations
TL;DR: In this paper, a two-dimensional thin-layer chromatography (TLC) was developed that separated rat liver phosphatides into several phosphate-positive spots in about two hours developing time.
314 citations
260 citations