A study on the curative effect of nobiletin on paraquat induced toxicity in rat
TL;DR: In this paper, the authors evaluated the curative impact of nobiletin (NOB) on paraquat induced mitochondrial impairment in rats' hepatic tissues and found that NOB treatment potentially reduced the damaging effects of the PQ in the mitochondria isolated from hepatic tissue.
About: This article is published in Journal of King Saud University - Science.The article was published on 2022-01-01 and is currently open access. It has received 1 citations till now. The article focuses on the topics: Paraquat & Biochemistry.
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TL;DR: In this article , the advancements in graphene and its derivatives-based electrochemical sensors for analysis of food pollutants were summarized, and the results of pollutant detection in food samples by the graphene-based electrode have also been outlined.
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TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.
24,847 citations
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TL;DR: In this article, the catalytic activity of catalase has been investigated using ultraviolet (UV) spectrophotometry and Titrimetric methods, which is suitable for comparative studies for large series of measurements.
Abstract: Publisher Summary Catalase exerts a dual function: (1) decomposition of H 2 O 2 to give H 2 O and O 2 (catalytic activity) and (2) oxidation of H donors, for example, methanol, ethanol, formic acid, phenols, with the consumption of 1 mol of peroxide (peroxide activity) The kinetics of catalase does not obey the normal pattern Measurements of enzyme activity at substrate saturation or determination of the K s is therefore impossible In contrast to reactions proceeding at substrate saturation, the enzymic decomposition of H 2 O 2 is a first-order reaction, the rate of which is always proportional to the peroxide concentration present Consequently, to avoid a rapid decrease in the initial rate of the reaction, the assay must be carried out with relatively low concentrations of H 2 O 2 (about 001 M) This chapter discusses the catalytic activity of catalase The method of choice for biological material, however, is ultraviolet (UV) spectrophotometry Titrimetric methods are suitable for comparative studies For large series of measurements, there are either simple screening tests, which give a quick indication of the approximative catalase activity, or automated methods
20,238 citations
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01 Jan 1963
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Abstract: Methods of enzymatic analysis , Methods of enzymatic analysis , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
18,100 citations
TL;DR: Fixed-time assay measuring H 2 O 2 consumption and continuous monitoring of Glutathione disulfide (GSSG) formation are cited here.
Abstract: Publisher Summary To determine glutathione peroxidase reliably, some factors of potential pitfall have to be considered, for example, enzymatic side reactions of substrates (especially when crude tissue samples are assayed), high and variable spontaneous reaction rates of substrates, and the peculiar kinetics of the enzyme itself. With the best documented example, the enzyme of bovine red blood cells, ping-pong kinetics with infinite limiting maximum velocities, and Michaelis constants have been established. This means that the generally recommended conditions for determination of enzyme activity––that is, “saturating” concentrations of all substrates, cannot possibly be fulfilled. In consequence, compromises are inevitable in the choice of substrate concentration for the assay and in the definition of the unit of activity. Fixed-time assay measuring H 2 O 2 consumption and continuous monitoring of Glutathione disulfide (GSSG) formation are cited here. The main differences between the assay procedure described and those proposed by others are listed in the chapter. To compare the results obtained by different procedures, appropriate empirical converting factors are also given.
4,341 citations
TL;DR: The activities of some glutathione-metabolizing enzymes were observed to be 5-to 60-fold lower in lung tissue than in the liver, and that phenobarbital nor methylcholanthrene had a significant effect on the levels of reduced glutATHione in lung and liver.
3,842 citations