Fig. 2 R1FL-Cys4 / R1FL-(Cys4)2 labeling in N2a cells using bmercaptoethanol: N2a cells were transiently transfected with R1FL-Cys4 (a), R1FL-(Cys4)2 (b) or R1FL-EGFP (c). R1FLCys4/R1FL-(Cys4)2 expressing cells were stained with 0.3 lM FlAsH/Lumio Green + 1.2 lM 2-ME with a subsequent wash with 100 lM 2-ME and analyzed by fluorescence microscopy. All bars, 10 lm
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