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Journal ArticleDOI

Accurate characterization of pure silicon-substituted hydroxyapatite powders synthesized by a new precipitation route

TL;DR: The results, particularly those from infrared spectroscopy, raise serious reservations about the phase purity of previously prepared and biologically evaluated SiHA powders, pellets and scaffolds in the literature.
About: This article is published in Acta Biomaterialia.The article was published on 2013-06-01 and is currently open access. It has received 88 citations till now. The article focuses on the topics: Infrared spectroscopy & Fourier transform infrared spectroscopy.

Summary (1 min read)

1. Introduction

  • In order to correctly describe the physical, chemical and biological properties of SiHAs and to compare them to routinely implanted HA and β-TCP, well-characterized pure SiHAs powders first need to be prepared.
  • Therefore, this work was devoted to the development of a new route to synthesize monophasic SiHA powders with controlled stoichiometry.
  • To this purpose, a solution of soluble silicate was first prepared from TEOS via a sol-gel route, and then accurate powder analysis was carried out by means of ICP/AES, Xray powder diffraction, Rietveld refinement, high resolution electron transmission microscopy (HR-TEM) with energy dispersive spectroscopy (EDS) as well as infrared (FT-IR/ATR) and solid-state NMR spectroscopy.
  • Two pH levels of precipitation were studied, as well as six Si/P molar ratios.

2.1 Powder synthesis

  • The as-synthesized powders were heated under air using an alumina crucible.
  • The heating and cooling rate was fixed at 4°C min -1 .

2.2.1 X-ray powder diffraction and Rietveld refinement

  • Crystalline phases were identified by means of a Siemens D5000 θ/2θ X-ray diffractometer.
  • The evolution of the crystallinity of the samples after calcination at 1000°C for 15 h was evaluated by means of the full width at half maximum (FWHM) of the (211) peak at 2θ=31.8°, as it had the highest intensity and minimal overlap with neighboring peaks.

2.2.4 Electron microscopy (HR-TEM, SAED and EDX)

  • Gold was then distributed as crystallized nano-domains which were used as a reference in the selected area electron diffraction (SAED) patterns to calculate as precisely as possible the lattice parameters.
  • The SAED patterns obtained from regions with or without gold on the HA part were the same.

3.2.4 Electron microscopy

  • The results are the average of about ten intervals per pattern.
  • Moreover, other experimental patterns for different zone axes (not shown here) were obtained and compared to theoretical electron diffraction patterns calculated by means of the Java Electron Microscopy Simulation (JEMS) software [87] .
  • The results indicate that the experimental and simulated patterns are perfectly superimposed for 0.734 ≥ c/a ≥ 0.729.

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Citations
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Journal ArticleDOI
TL;DR: This review discusses the current data obtained from original research in biochemistry and biomaterials science supporting the role of silicon in bone, comparing both the biological function of the element and analysing the evolution of silicon-containing biommaterials.

150 citations

Book ChapterDOI
01 Jan 2015

100 citations

Journal ArticleDOI
TL;DR: The present review is mainly focused on both the capability of HAp nanoparticles to encapsulate diverse compounds as well as the preparation methods of scaffolds incorporating HAp.
Abstract: Composites of hydroxyapatite (HAp) are widely employed in biomedical applications due to their biocompatibility, bioactivity and osteoconductivity properties. In fact, the development of industrially scalable hybrids at low cost and high efficiency has a great impact, for example, on bone tissue engineering applications and even as drug delivery systems. New nanocomposites constituted by HAp nanoparticles and synthetic or natural polymers with biodegradable and biocompatible characteristics have constantly been developed and extensive works have been published concerning their applications. The present review is mainly focused on both the capability of HAp nanoparticles to encapsulate diverse compounds as well as the preparation methods of scaffolds incorporating HAp. Attention has also been paid to the recent developments on antimicrobial scaffolds, bioactive membranes, magnetic scaffolds, in vivo imaging systems, hydrogels and coatings that made use of HAp nanoparticles.

83 citations

Journal ArticleDOI
TL;DR: This review focuses on state-of-the-art and the current advances in the development of 3D culture systems for bone biology research, and details main characteristics and challenges associated with its three main components, that is, scaffold, cells, and perfusion bioreactor systems.
Abstract: Most of our knowledge of bone cell physiology is derived from experiments carried out in vitro on polystyrene substrates However, these traditional monolayer cell cultures do not reproduce the complex and dynamic 3-dimensional (3D) environment experienced by cells in vivo Thus, there is a growing interest in the use of 3D culture systems as tools for understanding bone biology These in vitro engineered systems, less complex than in vivo models, should ultimately recapitulate and control the main biophysical, biochemical and biomechanical cues that define the in vivo bone environment, while allowing their monitoring This review focuses on state of the art and the current advances in the development of 3D culture systems for bone biology research It describes more specifically advantages related to the use of such systems, and details main characteristics and challenges associated with its three main components, ie scaffold, cells and perfusion bioreactor systems Finally, future challenges for non-invasive imaging technologies are addressed

80 citations

Journal ArticleDOI
01 Jun 2021
TL;DR: In this paper, a review summarises recent and relevant studies on cationic and anionic substitutions in the HAp lattice that are commonly found in the human body.
Abstract: Biological apatites are characterised by various ionic substitutions within the HAp lattice that are crucial for bone metabolism. The introduction of key role elements within synthetic calcium phosphates (CaP), mainly hydroxyapatite (HAp), can increase osteogenesis and enhance bone regeneration process. The lattice structure of HAp enables cationic and anionic substitutions leading to the enhanced biological performance of synthetic bone graft materials. This review summarises recent and relevant studies on cationic and anionic substitutions in the HAp lattice that are commonly found in the human body. Furthermore, co-substituted HAp obtained from synthetic and biological precursors, along with their influence on the bone regeneration process, has been discussed. Finally, future perspectives for the use of substituted HAp have been presented.

77 citations

References
More filters
Book
18 Jun 2004
TL;DR: This new edition of this highly successful manual is not only a revised text but has been extended to meet the interpretive needs of Raman users as well as those working in the IR region, creating a uniquely practical, comprehensive and detailed source for spectral interpretation.
Abstract: The third edition of this highly successful manual is not only a revised text but has been extended to meet the interpretive needs of Raman users as well as those working in the IR region. The result is a uniquely practical, comprehensive and detailed source for spectral interpretation. Combining in one volume, the correlation charts and tables for spectral interpretation for these two complementary techniques, this book will be of great benefit to those using or considering either technique.In addition to the new Raman coverage the new edition offers:* new section on macromolecules including synthetic polymers and biomolecules;* expansion of the section on NIR (near infrared region) to reflect recent growth in this area;* extended chapter on inorganic compounds including minerals and glasses;* redrawn and updated charts plus a number of new charts covering data new to this edition.This new edition will be invaluable in every industrial, university, government and hospital laboratory where infrared (FT-IR) and Raman spectral data need to be analysed.

6,428 citations

Book
01 Jan 1994
TL;DR: In this paper, the authors describe the structure of carbonate apatites and the properties of apatite powders, including their properties in terms of their nucleation and crystal growth.
Abstract: 1. General Chemistry of the Calcium Orthophosphates. Introduction. Monocalcium phosphates (monohydrate and anhydrous). Octacalcium phosphate. Dicalcium phosphate dehydrate, brushite. Dicalcium phosphate anhydrous, monetite. Anhydrous tricalcium phosphates and whitlockite. Tetracalcium phosphate. Amorphous calcium phosphates. 2. Fluorapatite and chlorapatite. Introduction. Structures. Substitutions in apatites. Preparation of powders. Growth of single crystals. Infrared and Raman spectra. Other physical and chemical studies. 3. Hydroxapatite and Nonstoichiometric Apatites. Introduction. Structure of hydroxyapatite. Preparation of stoichiometric hydroxyapatite powders. Preparation of other apatites with hydroxyl ions. Growth of hydroxyapatite single crystals. Special analytical methods. Structure of calcium-deficient hydroxyapatites. Kinetics of nucleation and crystal growth. Solubility and interfacial phenomena. Reactions in solution. Infrared and Raman spectroscopy. NMR spectroscopy. Other physical and chemical studies. 4. Mineral, synthetic and biological carbonate apatites. Introduction. Francolite and dahllite. A-type carbonate apatite, Caio(PO4)6CO3. Synthetic high temperature B-type carbonate apatites. Carbonate apatites from aqueous systems. Biological apatites. Electron spin resonance of X-irradiated carbonate apatites. Summary. Appendix. Calculated X-ray diffraction patterns of the calcium orthophosphates. References. Index.

2,352 citations


"Accurate characterization of pure s..." refers background or methods in this paper

  • ...0 powders mainly present bands characteristic of 432 hydroxyapatite with the ν1 (962 cm ), ν2 (473 cm ), ν3 (1021 and 1085 cm ) and ν4 (562 and 433 600 cm) modes of PO4 , as well as the stretching ( νS: 3572 cm ) and librational ( νL: 629 cm 434 (1)) modes of hydroxide groups [81, 92, 94]....

    [...]

  • ...and ν4), and the stretching ( νS) and librational ( νL) modes of the hydroxide groups [81, 92-94]....

    [...]

  • ...The former was chosen 182 according to phosphate and silicate speciation curv es, in order to have HPO 4 2- [81] and 183 H3SiO4 - ions [82] in solution as the main phosphate and si licate species, respectively....

    [...]

Journal ArticleDOI
16 Jan 1970-Science
TL;DR: Silicon, a relatively unknown trace element in nutritional research, has been uniquely localized in active calcification sites in young bone and is suggested to be associated with calcium in an early stage of calcification.
Abstract: Silicon, a relatively unknown trace element in nutritional research, has been uniquely localized in active calcification sites in young bone. Silicon increases directly with calcium at relatively low calcium concentrations and falls below the detection limit at compositions approaching hydroxyapatite. It is suggested that silicon is associated with calcium in an early stage of calcification.

867 citations


"Accurate characterization of pure s..." refers background in this paper

  • ...Two other SAED patterns were 324 obtained for the [1-10] zone axis....

    [...]

  • ...established that silicon plays an important role in the early stage of cartilage and bone growth 52 [1-4]....

    [...]

Journal ArticleDOI
01 Feb 2003-Bone
TL;DR: In conclusion, orthosilicic acid at physiological concentrations stimulates collagen type 1 synthesis in human osteoblast-like cells and enhances osteoblastic differentiation.

773 citations


"Accurate characterization of pure s..." refers background in this paper

  • ...Two other SAED patterns were 324 obtained for the [1-10] zone axis....

    [...]

  • ...collagen by human osteoblast cells (MG-63 cell line ) vitro [7]....

    [...]

Journal ArticleDOI
TL;DR: The subject of hydrogen bonding plays a significant role in determining the structure of crystals and hence the subject is of much interest to physicists, chemists and molecular biologists as discussed by the authors, and as there is now considerable agreement in recognizing hydrogen bonding experimentally, it is also timely.
Abstract: W. C. Hamilton and J. A. Ibers New York: W. A. Benjamin Inc. 1968 Pp. xv+ 284. Price $13.95. Hydrogen bonding plays a significant role in determining the structure of crystals and hence the subject is of much interest to physicists, chemists and molecular biologists. This book should serve their needs for a concise text admirably, and as there is now considerable agreement in recognizing the occurrence of hydrogen bonding experimentally, it is also timely.

752 citations

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