Journal ArticleDOI
Acidocalcisomes ? conserved from bacteria to man
Roberto Docampo,Roberto Docampo,Wanderley de Souza,Kildare Miranda,Kildare Miranda,Peter Rohloff,Silvia N.J. Moreno,Silvia N.J. Moreno +7 more
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TLDR
Acidocalcisomes were first described in trypanosomatids and have been characterized in most detail in these species, and are linked with several functions, including storage of cations and phosphorus, polyphosphate metabolism, calciumHomeostasis, maintenance of intracellular pH homeostasis and osmoregulation.Abstract:
Recent work has shown that acidocalcisomes, which are electron-dense acidic organelles rich in calcium and polyphosphate, are the only organelles that have been conserved during evolution from prokaryotes to eukaryotes Acidocalcisomes were first described in trypanosomatids and have been characterized in most detail in these species Acidocalcisomes have been linked with several functions, including storage of cations and phosphorus, polyphosphate metabolism, calcium homeostasis, maintenance of intracellular pH homeostasis and osmoregulation Here, we review acidocalcisome ultrastructure, composition and function in different trypanosomatids and other organismsread more
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Journal ArticleDOI
Inorganic polyphosphate: essential for growth and survival.
TL;DR: There is extensive evidence that the remarkable properties of Poly P as a polyanion have made it suited for a crucial role in the emergence of cells on earth, and this review pays particular attention to the enzyme, polyphosphate kinase 1, responsible for Poly P synthesis and highly conserved in many bacterial species, including 20 or more of the major pathogens.
Journal ArticleDOI
Polyphosphate modulates blood coagulation and fibrinolysis
Stephanie A. Smith,Nicola J. Mutch,Deepak Baskar,Peter Rohloff,Roberto Docampo,Roberto Docampo,James H. Morrissey +6 more
TL;DR: It is reported that polyphosphate is a potent hemostatic regulator, accelerating blood clotting by activating the contact pathway and promoting the activation of factor V, which in turn results in abrogation of the function of the natural anticoagulant protein, tissue factor pathway inhibitor.
Journal ArticleDOI
Specific chemotherapy of Chagas disease: relevance, current limitations and new approaches.
TL;DR: A critical review of the development of specific chemotherapeutic approaches for the management of American Trypanosomiasis or Chagas disease is presented, and the most promising approaches are ergosterol biosynthesis inhibitors such as posaconazole and ravuconazole.
Journal ArticleDOI
Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease
TL;DR: The role of the Ca2+-mobilizing messenger NAADP (nicotinic acid adenine dinucleotide phosphate) as a major regulator of Ca2+, and the recent discovery of an endolysosomal channel family, the TPCs (two-pore channels), as its principal intracellular targets are discussed.
Journal ArticleDOI
Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase.
Michael Hothorn,Heinz Neumann,Esther D. Lenherr,Mark Wehner,Vladimir Rybin,Paul O. Hassa,Andreas Uttenweiler,Monique Reinhardt,Andrea Schmidt,Jeanette Seiler,Andreas G. Ladurner,Christian Herrmann,Klaus Scheffzek,Andreas Mayer,Andreas Mayer +14 more
TL;DR: Using x-ray crystallography, a eukaryotic polyphosphate polymerase is identified within the membrane-integral vacuolar transporter chaperone (VTC) complex and mutational analysis of the transmembrane domain indicates that VTC may integrate cytoplasmic polymer synthesis with polyP membrane translocation.
References
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Leishmaniasis– current chemotherapy and recent advances in the search for novel drugs
Simon L. Croft,Graham H. Coombs +1 more
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Journal ArticleDOI
Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.
TL;DR: It is concluded that four extra, reading frame-restoring nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process.
Book ChapterDOI
Inorganic Polyphosphate: A Molecule of Many Functions
TL;DR: In this article, the authors focus on recent advances made possible by the introduction of novel, enzymatically based assays, such as Escherichia coli polyphosphate kinase (PPK) that can convert poly P and ADP to ATP and yeast exopolyphosphatase that can hydrolyze poly P to Pi.