Acinetobacter nectaris sp. nov. and Acinetobacter boissieri sp. nov., isolated from floral nectar of wild Mediterranean insect-pollinated plants
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Cites background from "Acinetobacter nectaris sp. nov. and..."
...Several species among them are known to be methylotrophic [30–32], and there are many reports on their association with plants [33–39], although the genus is generally known as non-motile [33,40,41]....
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6 citations
Cites background from "Acinetobacter nectaris sp. nov. and..."
...Strains: 1, GVCNT2 (data from this study); 2, Acinetobacter calcoaceticus DSM 30006 (Álvarez-Pérez et al., 2013; Song et al., 2008), 3, Alkanindiges illinoisensis DSM 15370 (Bogan et al....
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...…strain GVCNT2T and other members of the family Moraxellaceae Strains: 1, GVCNT2T (data from this study); 2, Acinetobacter calcoaceticus DSM 30006T (Álvarez-Pérez et al., 2013; Song et al., 2008), 3, Alkanindiges illinoisensis DSM 15370T (Bogan et al., 2003; Song et al., 2008); 4, Enhydrobacter…...
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References
39,110 citations
"Acinetobacter nectaris sp. nov. and..." refers methods in this paper
...Following these procedures, 1320 nt positions (98 % of the original alignment) remained for subsequent phylogenetic analysis using the neighbour-joining (NJ) method as implemented in the MEGA 5 software package (Tamura et al., 2011)....
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8,757 citations
"Acinetobacter nectaris sp. nov. and..." refers methods in this paper
...0 (Hall, 1999) to ensure that all sequences had the same start and end point, and analysed with Gblocks (Castresana, 2000) to eliminate ambiguously aligned regions, using ‘allow gap positions5with half’, ‘minimum length of a block559 and default settings for all other options....
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...…alignment was trimmed with BioEdit v. 7.0.9.0 (Hall, 1999) to ensure that all sequences had the same start and end point, and analysed with Gblocks (Castresana, 2000) to eliminate ambiguously aligned regions, using ‘allow gap positions5with half’, ‘minimum length of a block559 and default settings…...
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...As with the 16S rRNA gene sequence, concatenated sequences of rpoB zones 1 and 2 of the nectar strains and type strains of other species of the genus Acinetobacter were included in a multiple alignment and analysed with Gblocks, which resulted in the selection of 843 nucleotide positions (99 % of the original alignment)....
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...The resulting alignment was trimmed with BioEdit v. 7.0.9.0 (Hall, 1999) to ensure that all sequences had the same start and end point, and analysed with Gblocks (Castresana, 2000) to eliminate ambiguously aligned regions, using ‘allow gap positions5with half’, ‘minimum length of a block559 and default settings for all other options....
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5,300 citations
"Acinetobacter nectaris sp. nov. and..." refers methods in this paper
...The 16S rRNA gene sequences of the novel nectar strains and reference strains of members of the genus Acinetobacter and the family Moraxellaceae were included in a multiple alignment generated by CLUSTAL W (Chenna et al., 2003)....
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4,685 citations
"Acinetobacter nectaris sp. nov. and..." refers methods in this paper
...The DNA was enzymically degraded into nucleosides and the nucleotidic base composition was determined by HPLC, according to the method of Mesbah et al. (1989)....
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