Journal ArticleDOI
Active urea transport and an unusual basolateral membrane composition in the gills of a marine elasmobranch
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TLDR
This work proposes that a unique combination of active urea transport and modification of the phospholipid bilayer membrane is responsible for decreasing the gill permeability to urea and facilitating urea retention by the gills of Squalus acanthias.Abstract:
In elasmobranch fishes, urea occurs at high concentrations (350–600 mM) in the body fluids and tissues, where it plays an important role in osmoregulation. Retention of urea by the gill against thi...read more
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The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste
TL;DR: The fish gill is a multipurpose organ that, in addition to providing for aquatic gas exchange, plays dominant roles in osmotic and ionic regulation, acid-base regulation, and excretion of nitrogenous wastes.
Journal ArticleDOI
Ammonia toxicity in fish
D.J Randall,T.K.N Tsui +1 more
TL;DR: Fish have strategies to protect them from the ammonia pulse following feeding, and this also protects them from increases in external ammonia, as a result starved fish are more sensitive to external ammonia than fed fish.
Journal ArticleDOI
Ammonia production, excretion, toxicity, and defense in fish: a review.
Yuen K. Ip,Shit F. Chew +1 more
TL;DR: This review focuses on both the earlier literature and the up-to-date information on the problems and mechanisms concerning the permeation of ammonia across mitochondrial membranes, the blood–brain barrier, the plasmalemma of neurons, and the branchial and cutaneous epithelia of fish.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
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Thermal adaptation in biological membranes: is homeoviscous adaptation the explanation?
TL;DR: HV A has emerged as the most commonly employed paradigm to assess the efficacy of thermal adaptation in biological membranes and to explain patterns of temperature-induced change in membrane lipid composi-
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Methods for Nonlethal Gill Biopsy and Measurement of Na+, K+-ATPase Activity
TL;DR: A gill biopsy, in which a small portion of gill tissue was removed from anesthetized fish, was shown to have no detrimental effect on subsequent survival, growth, and salinity tolerance of juvenile fish.