Adhesion of osteoblasts to a nanorough titanium implant surface

doi:10.2147/IJN.S21755

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Adhesion of osteoblasts to a nanorough titanium implant surface

Journal Article•DOI•

Adhesion of osteoblasts to a nanorough titanium implant surface

Ekaterina Gongadze¹, Doron Kabaso, Sebastian Bauer, Tomaž Slivnik, Patrik Schmuki, Ursula van Rienen, Aleš Iglič² - Show less +3 more•Institutions (2)

University of Rostock¹, University of Ljubljana²

31 Aug 2011-International Journal of Nanomedicine (Dove Press)-Vol. 6, pp 1801-1816

TL;DR: It is suggested that osteoblasts are most strongly bound along the sharp convex edges or spikes of nanorough titanium surfaces where the magnitude of the negative surface charge density is the highest and it is plausible that nanorough regions of titanium surfaces with sharp edges and spikes promote the adhesion of osteoblast.

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Abstract: This work considers the adhesion of cells to a nanorough titanium implant surface with sharp edges. The basic assumption was that the attraction between the negatively charged titanium surface and a negatively charged osteoblast is mediated by charged proteins with a distinctive quadrupolar internal charge distribution. Similarly, cation-mediated attraction between fibronectin molecules and the titanium surface is expected to be more efficient for a high surface charge density, resulting in facilitated integrin mediated osteoblast adhesion. We suggest that osteoblasts are most strongly bound along the sharp convex edges or spikes of nanorough titanium surfaces where the magnitude of the negative surface charge density is the highest. It is therefore plausible that nanorough regions of titanium surfaces with sharp edges and spikes promote the adhesion of osteoblasts.

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Journal Article•DOI•

One-dimensional titanium dioxide nanomaterials: nanotubes.

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Kiyoung Lee¹, Anca Mazare¹, Patrik Schmuki¹•Institutions (1)

University of Erlangen-Nuremberg¹

14 Aug 2014-Chemical Reviews

TL;DR: The present review tries to give a comprehensive and most up to date view to the field, with an emphasis on the currently most investigated anodic TiO2 nanotube arrays.

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Abstract: In the present review we try to give a comprehensive and most up to date view to the field, with an emphasis on the currently most investigated anodic TiO2 nanotube arrays. We will first give an overview of different synthesis approaches to produce TiO2 nanotubes and TiO2 nanotube arrays, and then deal with physical and chemical properties of TiO2 nanotubes and techniques to modify them. Finally, we will provide an overview of the most explored and prospective applications of nanotubular TiO2.

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984 citations

Journal Article•DOI•

Titanium nanostructures for biomedical applications

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Mukta Kulkarni¹, Anca Mazare¹, Ekaterina Gongadze², Šárka Perutková², Veronika Kralj-Iglič², Ingrid Milošev³, Patrik Schmuki¹, Aleš Iglič², Miran Mozetič³ - Show less +5 more•Institutions (3)

University of Erlangen-Nuremberg¹, University of Ljubljana², Jožef Stefan Institute³

22 Jan 2015-Nanotechnology

TL;DR: Perhaps the most spectacular and surprising one-dimensional structures and their unique biomedical applications for increased osseointegration, protein interaction and antibacterial properties are focused on.

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Abstract: Titanium and titanium alloys exhibit a unique combination of strength and biocompatibility, which enables their use in medical applications and accounts for their extensive use as implant materials in the last 50 years. Currently, a large amount of research is being carried out in order to determine the optimal surface topography for use in bioapplications, and thus the emphasis is on nanotechnology for biomedical applications. It was recently shown that titanium implants with rough surface topography and free energy increase osteoblast adhesion, maturation and subsequent bone formation. Furthermore, the adhesion of different cell lines to the surface of titanium implants is influenced by the surface characteristics of titanium; namely topography, charge distribution and chemistry. The present review article focuses on the specific nanotopography of titanium, i.e. titanium dioxide (TiO2) nanotubes, using a simple electrochemical anodisation method of the metallic substrate and other processes such as the hydrothermal or sol-gel template. One key advantage of using TiO2 nanotubes in cell interactions is based on the fact that TiO2 nanotube morphology is correlated with cell adhesion, spreading, growth and differentiation of mesenchymal stem cells, which were shown to be maximally induced on smaller diameter nanotubes (15 nm), but hindered on larger diameter (100 nm) tubes, leading to cell death and apoptosis. Research has supported the significance of nanotopography (TiO2 nanotube diameter) in cell adhesion and cell growth, and suggests that the mechanics of focal adhesion formation are similar among different cell types. As such, the present review will focus on perhaps the most spectacular and surprising one-dimensional structures and their unique biomedical applications for increased osseointegration, protein interaction and antibacterial properties.

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395 citations

Journal Article•DOI•

TiO2 nanotubes, nanochannels and mesosponge: Self-organized formation and applications

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Damian Kowalski¹, Doohun Kim², Patrik Schmuki¹, Patrik Schmuki³•Institutions (3)

University of Erlangen-Nuremberg¹, Korea Electrotechnology Research Institute², King Abdulaziz University³

01 Jun 2013-Nano Today

TL;DR: In this article, a review of recent advances in the formation of nanostructured oxides in the form of nanotubes, nanopores with a through-hole morphology, mesosponges, nanochannels and microcones grown on Ti, Nb, Ta, Zr, Hf, W, V and their alloys is presented.

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309 citations

Journal Article•DOI•

Binary titanium alloys as dental implant materials-a review.

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Xiaotian Liu¹, Xiaotian Liu², Shuyang Chen¹, Shuyang Chen², James K.H. Tsoi¹, Jukka Pekka Matinlinna¹ - Show less +2 more•Institutions (2)

University of Hong Kong¹, Nankai University²

23 Sep 2017-Regenerative Biomaterials

TL;DR: Most of the binary Ti alloys with alloying <20% elements of Zr, In, Ag, Cu, Au, Pd, Nb, Mn, Cr, Mo, Sn and Co have high potential as implant materials, due to good mechanical performance without compromising the biocompatibility and biological behaviour compare to cp-Ti.

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Abstract: Titanium (Ti) has been used for long in dentistry and medicine for implant purpose During the years, not only the commercially pure Ti but also some alloys such as binary and tertiary Ti alloys were used The aim of this review is to describe and compare the current literature on binary Ti alloys, including Ti-Zr, Ti-In, Ti-Ag, Ti-Cu, Ti-Au, Ti-Pd, Ti-Nb, Ti-Mn, Ti-Mo, Ti-Cr, Ti-Co, Ti-Sn, Ti-Ge and Ti-Ga, in particular to mechanical, chemical and biological parameters related to implant application Literature was searched using the PubMed and Web of Science databases, as well as google without limiting the year, but with principle key terms such as ' Ti alloy', 'binary Ti ', 'Ti-X' (with X is the alloy element), 'dental implant' and 'medical implant' Only laboratory studies that intentionally for implant or biomedical applications were included According to available literatures, we might conclude that most of the binary Ti alloys with alloying <20% elements of Zr, In, Ag, Cu, Au, Pd, Nb, Mn, Cr, Mo, Sn and Co have high potential as implant materials, due to good mechanical performance without compromising the biocompatibility and biological behaviour compare to cp-Ti

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164 citations

Cites background from "Adhesion of osteoblasts to a nanoro..."

...Thus, surface treatment methods such as the acid etching could not only roughen the surface that provide a harbouring site for negatively charged osteoblast [22], but also induce the formation of hydroxylated Ti oxide form [Ti-OH2] þ, which is hydrophilic and documented for biological activity enhancement [23]....
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Journal Article•DOI•

Tuning surface properties of bone biomaterials to manipulate osteoblastic cell adhesion and the signaling pathways for the enhancement of early osseointegration.

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Shoucheng Chen¹, Yuanlong Guo¹, Runheng Liu¹, Shiyu Wu¹, Jinghan Fang¹, Baoxin Huang¹, Zhipeng Li¹, Zhuofan Chen¹, Zetao Chen¹ - Show less +5 more•Institutions (1)

Sun Yat-sen University¹

01 Apr 2018-Colloids and Surfaces B: Biointerfaces

TL;DR: This review provided up-to-date research outcomes in the adhesion behavior of osteoblastic cells on bone biomaterials with different physicochemical properties and the proposed strategy that manipulating cell adhesion and the downstream signaling network for the enhancement of early osseointegration.

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137 citations

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Ulrich Hersel¹, Claudia Dahmen¹, Horst Kessler¹•Institutions (1)

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TL;DR: The impacts of RGD peptide surface density, spatial arrangement as well as integrin affinity and selectivity on cell responses like adhesion and migration are discussed.

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TL;DR: Current approaches to control cell behavior through the nanoscale engineering of materials surfaces are reviewed and implications are emerging for applications including medical implants, cell supports, and materials that can be used as instructive three-dimensional environments for tissue regeneration.

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Abstract: Cells are inherently sensitive to local mesoscale, microscale, and nanoscale patterns of chemistry and topography. We review current approaches to control cell behavior through the nanoscale engineering of materials surfaces. Far-reaching implications are emerging for applications including medical implants, cell supports, and materials that can be used as instructive three-dimensional environments for tissue regeneration.

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Journal Article•DOI•

Osteoblast adhesion on biomaterials.

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Karine Anselme

01 Apr 2000-Biomaterials

TL;DR: An understanding of the proteins involved in osteoblast adhesion opens up new possibilities for the grafting of these proteins (or synthesized peptide) onto vector materials, to increase their in vivo bioactivity or to promote cell integration within the vector material during the development of hybrid materials.

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2,361 citations

"Adhesion of osteoblasts to a nanoro..." refers background or result in this paper

...These showed that osteoblasts are flattened so closely onto the positively charged substrata that the ventral cell membrane was not distinguishable under the transmission electron microscope, while on negatively charged substrata the ventral cell membrane was readily visible only with focal contacts with the substrata.(3,5) This indicates that the osteoblasts are strongly bound to the positively charged substrate due to direct electrostatic binding of a negatively charged glycocalyx of an osteoblast membrane to the positive substrate, while the binding of an osteoblast to a negatively charged substrate is mediated by proteins with a distinctive quadrupolar internal charge distribution (Figure 2)....
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...In addition to fibronectin (considered in this work), human osteoblasts also adhere to vitronectin(6) and weakly to laminin.(3) The structure of vitronectin, an integrin binding protein, was predicted through a combination of computational methods and experimental approaches....
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...However, on rough surfaces, focal contacts were visible only at the extremities of the cell extensions where cell membranes were in contact with the substrate.(3,4) In the present study, lattice statistics of the equilibrium state was employed to demonstrate that in the limit of a large binding potential for integrins in a nanorough region (Eq....
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...Discussion and conclusions To understand the role of proteins (such as fibronectins, vitronectins, and laminins) involved in osteoblast adhesion on the surface of a titanium implant is of crucial importance.(3,6) Our basic assumption that the attraction between a negatively charged titanium surface and a negatively charged osteoblast is mediated by charged proteins with a distinctive quadrupolar internal charge distribution(15) may be supported by experimental results....
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...Like fibronectin, vitronectin also contains an Arg-Gly-Asp (RGD) sequence close to the somatomedin B domain which is responsible for integrin binding and cell adhesion.(3,53) The highly positively charged group of vitronectin near the C-terminal group on the other side of the molecule (largely uninterrupted by negative charges) is the binding site for the negatively charged molecules of heparin....
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