Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
Citations
756 citations
Cites methods from "Advancing uracil-excision based clo..."
...The cDNA of SLAC1, OST1, and ABI1 were cloned into plant binary vectors (based on pCAMBIA vectors) as described by Nour-Eldin et al. (2006) (63)....
[...]
659 citations
548 citations
Cites background from "Advancing uracil-excision based clo..."
...Finally, one could entertain the assembly of plasmids à la carte by recombining relevant DNA sequences in vitro with suitable enzymatic cocktails (25,26)....
[...]
493 citations
392 citations
References
18,757 citations
"Advancing uracil-excision based clo..." refers methods in this paper
...Arabidopsis thaliana plants were transformed by the floral dip method (7)....
[...]
2,830 citations
"Advancing uracil-excision based clo..." refers background or methods in this paper
...laevis oocytes T7 For N-terminal fusions to YFP(17)....
[...]
...laevis oocytes T7 For C-terminal fusions to YFP (17)....
[...]
...laevis oocytes T7 For C-terminal fusions to the N-terminal part of YFP for use in BiFC (17,18)....
[...]
...With this approach, we inserted YFP (17), YFP-based bimolecular fluorescence complementation (BiFC) tags (18) and RGSHis6 tags into our existing USER vectors to construct a comprehensive set of C- and N-terminal translational fusion vectors for subcellular localization, protein–protein interaction and protein purification studies, respectively....
[...]
...laevis oocytes T7 For N-terminal fusions to the C-terminal part of YFP for use in BiFC (17,18)....
[...]
1,674 citations
1,547 citations
"Advancing uracil-excision based clo..." refers background or methods in this paper
...laevis oocytes T7 For C-terminal fusions to the N-terminal part of YFP for use in BiFC (17,18)....
[...]
...With this approach, we inserted YFP (17), YFP-based bimolecular fluorescence complementation (BiFC) tags (18) and RGSHis6 tags into our existing USER vectors to construct a comprehensive set of C- and N-terminal translational fusion vectors for subcellular localization, protein–protein interaction and protein purification studies, respectively....
[...]
...laevis oocytes T7 For N-terminal fusions to the C-terminal part of YFP for use in BiFC (17,18)....
[...]
...N-terminal part of YFP for use in BiFC (17,18)....
[...]
...C-terminal part of YFP for use in BiFC (17,18)....
[...]
800 citations
"Advancing uracil-excision based clo..." refers methods in this paper
...The resulting vector construct was subsequently transformed by electroporation (5) into Agrobacterium tumefaciens strain C58 (6)....
[...]