Journal ArticleDOI
Affinity chromatography: A versatile technique for antibody purification.
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TLDR
A detailed overview on affinity chromatography and the components involved in purification is provided to provide a detailed overview of antibody purification methodologies and their underlying working principles.About:
This article is published in Methods.The article was published on 2017-03-01. It has received 64 citations till now. The article focuses on the topics: Downstream processing.read more
Citations
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Journal ArticleDOI
Recent advances on protein separation and purification methods.
TL;DR: The progress of protein separation technology was reviewed from the aspects of extraction, precipitation, membrane separation, chromatography, electrophoresis, molecular imprinting, microfluidic chip and so on.
Journal ArticleDOI
Affinity chromatography: A review of trends and developments over the past 50 years
Elliott Rodriguez,Saumen Poddar,Sazia Iftekhar,Kyungah Suh,Ashley G. Woolfork,Susan T. Ovbude,Allegra Pekarek,Morgan Walters,Shae Lott,David S. Hage +9 more
TL;DR: This review examines the major developments and trends that have occurred in this technique over the past five decades and identifies major trends in these topics and important areas of use for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.
Journal ArticleDOI
Design, development and characterization of ACT017, a humanized Fab that blocks platelet's glycoprotein VI function without causing bleeding risks.
Kristell Lebozec,Martine Jandrot-Perrus,Gilles Avenard,Olivier Favre-Bulle,Philippe Billiald +4 more
TL;DR: It is demonstrated, in a dose-escalation study, that ACT017 has a high capacity to specifically inhibit collagen-induced platelet aggregation ex vivo after injection to the macaque without inducing thrombocytopenia, GPVI depletion or bleeding side effects as is the case for conventional anti-platelets.
Journal ArticleDOI
Oriented immobilization of antibodies onto sensing platforms- A critical review
TL;DR: This review provides more insights into the fabrication of high-efficiency immunosensor, and special attention has been devoted to the well-orientation of full-length IgGs onto the sensing platform.
Journal ArticleDOI
Oriented immobilization of antibodies onto sensing platforms - A critical review
TL;DR: In this paper , the authors classified the stepwise strategy into two parts based on the different noncovalent interactions, namely adhesive layer-mediated interaction onto homofunctional support and layer-free interaction onto heter-of-functional support (which displays several different functionalities on its surface that are capable to interact with IgGs).
References
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Journal ArticleDOI
Hydrophilic Gels for Biological Use
Oto Wichterle,D. Lím +1 more
TL;DR: In this article, the structural similarity with the tissue has not been emphasized although physiologically unfavourable effects were observed in most cases of application of normal type plastics in permanent contact with living tissues.
Journal ArticleDOI
Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
Journal ArticleDOI
Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides
TL;DR: Biologically active proteins and polypeptides can be coupled by various means to cellulose, starch and cross-linked polysaccharide gels such as ‘Sephadex’.
Book ChapterDOI
[31] Affinity chromatography
TL;DR: Inherent advantages of this method of purification are the rapidity and ease of a potentially single-step procedure, the rapid separation of the protein to be purified from inhibitors and destructive contaminants, such as proteases, and protection from denaturation during purification by active site ligand-stabilization of protein tertiary structure.