Aggregation of gamma-G3 proteins: relevance to the hyperviscosity syndrome.
J. D. Capra,H. G. Kunkel +1 more
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Researchers revealed a concentration- and temperature-dependent aggregation which was not encountered in 26 sera from patients with multiple myeloma involving other gammaG subgroups, and the gammaG3 cases, however, showed the syndrome at relatively low protein concentrations.Abstract:
Studies on the sera and isolated proteins from 14 patients with gammaG3 multiple myeloma revealed a concentration- and temperature-dependent aggregation which was not encountered in 26 sera from patients with multiple myeloma involving other gammaG subgroups. When the gammaG3 myeloma sera were diluted and characterized by analytical ultracentrifugation, complex formation was minimal. However, when these sera were examined undiluted, marked complex formation was observed. Studies on the isolated proteins, their enzymatic fragments, as well as their heavy and light polypeptide chains localized the aggregating sites to the Fd fragment of the heavy chains. The findings were also documented by acrylamide-gel electrophoresis and capillary tube viscometry.THE HYPERVISCOSITY SYNDROME WAS OBSERVED IN SIX PATIENTS: three with gammaG3 myeloma and three with gammaG1 myeloma. In the latter group extreme protein concentrations appeared essential for the development of the clinical symptoms. The gammaG3 cases, however, because of the aggregation phenomenon, showed the syndrome at relatively low protein concentrations.read more
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Journal Article
Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion.
TL;DR: A gel diffusion method for demonstrating precipitin reactions of C1q with aggregated γ-globulin and immune complexes is described and the relevance of this in vitro system to in vivo complement consumption in various disease states is discussed.
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Proliferative Glomerulonephritis with Monoclonal IgG Deposits
Samih H. Nasr,Anjali A. Satoskar,Glen S. Markowitz,Anthony M. Valeri,Gerald B. Appel,Michael B. Stokes,Tibor Nadasdy,Vivette D. D'Agati +7 more
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Book ChapterDOI
Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes.
J B Natvig,H G Kunkel +1 more
TL;DR: The immunoglobulin classes and subclasses represent a group of structurally related proteins, and in all instances consist of two pairs of polypeptide chains held together by disulfide bridges and noncovalent forces.
Patent
Reduced-viscosity concentrated protein formulations
Jun Liu,Steven J. Shire +1 more
TL;DR: In this paper, a method for reducing the viscosity of concentrated protein formulations was proposed for subcutaneous administration, which is particularly suitable for sub-cutaneous drug administration.
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