Book ChapterDOI
Agrobacterium-Mediated Transformation for Insect-Resistant Plants
B. Sunil Kumar,C. Immanuel Selvaraj +1 more
- pp 275-283
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TLDR
Bacillus thuringiensis (Bt) is considered as gram-positive, aerobic, spore-forming, naturally occurring facultative soilborne bacterial pathogen and has been used for natural insect control and produces a parasporal, persistent insecticidal protein crystals (ICPs).Abstract:
Bacillus thuringiensis (Bt) is considered as gram-positive, aerobic, spore-forming, naturally occurring facultative soilborne bacterial pathogen and has been used for natural insect control. It produces a parasporal, persistent insecticidal protein crystals (ICPs). These ICPs are toxic in nature for a class of lepidopterans, dipterans, and coleopterans. That toxic protein differs, depending on the subspecies of Bt producing it. The most prevalent ICPs are the Cry (crystal) protein, and the other is Cty (cytolytic) protein produced by some Bacillus thuringiensis stains. The Cry proteins, in general were cleaved by proteolytic enzymes on intake to produce active toxins which results in osmotic imbalance, lysis of epithelial cells, and finally death due to starvation, whereas Cty proteins release vegetative insecticidal proteins (VIPs) which lead to membrane disruptions, midgut lysis, and paralysis in lepidopterans pests. The use of ICPs as a pesticide or insecticide over chemicals is more beneficial as there is less amount of environmental pollution and harmful chemical residues leaching into the soil and water bodies. It is also target specific and acts on specific class of pests and at the same time harmless to birds, fish, and mammals whose acidic gut conditions negate the bacteria’s effect.read more
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Book ChapterDOI
The Biotechnological Story of Microbial Genes from Soil to Transgenic Plants
TL;DR: A review of microbial genes-based transgenic plants for tolerance against abiotic stresses, improved nutrients, and disease resistance can be found in this paper , where the expression of foreign genes induce changes at the biochemical, physio-chemical, anatomical, morphological, and physiological levels, which ultimately improve stresses tolerance and crop production.
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Journal ArticleDOI
Efficient transformation of Korean rice cultivars (Oryza sativa L) mediated byAgrobacterium tumefaciens
Sung Ki Cho,Young Soo Chung,Sung Jin Park,Jeong Sheop Shin,Hyok Ji Kwon,Kyung Ho Kang,Kyung Ho Kang +6 more
TL;DR: It is suggested that the anther-derived calli are better-materials for transformation experiment due to their genotype-independent regeneration.