Aloe barbadensis Miller peptide/polypeptide fraction alleviates inflammation through inhibition of proinflammatory cytokines and mediators in vitro and in rats with Freund’s adjuvant-induced hind paw edema
01 Dec 2019-Asian pacific Journal of Tropical Biomedicine (Medknow Publications)-Vol. 9, Iss: 12, pp 524
TL;DR: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
Abstract: Objective: To evaluate the anti-inflammatory potential of peptide/polypeptide fraction of Aloe vera through in vitro and in vivo studies. Methods: The peptide/polypeptide fraction from Aloe vera was obtained through trichloroacetic acid precipitation. The anti-inflammatory property of the peptide/polypeptide fraction was tested by protein denaturation, membrane stabilization assays. The effect of the fraction on RAW 264.7 cell viability was examined by MTT assays. The nitric oxide level was determined through Griess reagent. TNF-α and IL-6 levels were estimated using ELISA kits. In vivo studies were carried out in male Wistar rats through injection of Freund’s adjuvant in the hind paw. Paw edema was measured through the Vernier scale and levels of alanine aminotransferase, aspartate transaminase, TNF-α, IL-6, and secretory phospholipase A2 were estimated through their respective kits after fourteen days of treatment. GraphPad Prism6 was used for analyzing the results. Results: The peptide/polypeptide extract inhibited protein denaturation with an IC50 value of (218.9±15.6) μg/mL and stabilized the membrane of red blood cells with an IC50 value of (275.9±19.1) μg/mL. The extract showed no changes in cell morphology or cytotoxicity up to the concentration of 20 μg/mL in MTT assays. The peptide/polypeptide fraction markedly reduced the levels of proinflammatory markers and mediators in both in vitro and in vivo studies. Conclusions: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
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TL;DR: In this article , immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.
23 citations
TL;DR: In this article, immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.
23 citations
TL;DR: This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits, and the potential applications and constraints have been discussed.
Abstract: The therapeutic and pharmacological properties of plant bioactive constituents still continue to be the subject of many researches. Species of the Aloe genus have a history in folklore medicine and they have gained more attention over the years due to their various medicinal properties. Phytochemical studies have revealed that the Aloe species contain a number of constituents, such as polyphenols, phytosterols, polysaccharides, proteins, amino acids, chromones, and mineral elements. A comprehensive evidence-based review on the different constituents of Aloe species is needed in order to understand the benefits imparted by them. This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits. This information will be beneficial for the advancement of new strategies of Aloe formulations with therapeutic and economical value in the near future. Furthermore, the potential applications and constraints have also been discussed so as to provide a wider prospect for research in this field for the benefit of the society.
11 citations
Cites background from "Aloe barbadensis Miller peptide/pol..."
...The flower grows around 90 cm, often being pendulous and consists of yellow tubular corolla around 2–3 cm (Babu et al., 2019)....
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...Apart from that, polyphenols from Aloe species have been reported to possess anti-diabetic potential, anti-inflammatory properties, and wound healing properties (Babu et al., 2019)....
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TL;DR: In this article, the mechanism of action of Aloe vera and its two constituents (Carbohydrates and Polypeptides) in the alleviation of diabetes in streptozotocin-induced diabetic rats through a proteomics approach was investigated.
7 citations
TL;DR: In this article, the role of peptide/polypeptide fraction (PPF) of Aloe vera in the alleviation of diabetes through maintaining the intestinal permeability by regulating the zonulin and GLP-1 levels.
7 citations
References
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TL;DR: The methods described in this review are not an exhaustive or comprehensive discussion of all methods available for the detection of NO but rather a description of the most commonly used and practical methods which allow accurate and sensitive quantification of NO products/metabolites in multiple biological matrices under normal physiological conditions.
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...The supernatant was collected and mixed with an equal amount of Griess reagent and NO levels were studied[20]....
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...However, these drugs have side effects like lesions, cardiovascular disorders, bleeding and also affect the gastrointestinal tract and immune system[3,4]....
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...Previous studies have reported the anti-inflammatory potential of Aloe vera gel, where the gel exerts its anti-inflammatory property through inhibition of COX2, prostaglandins, bradykinins[8] and through the inhibition of NF-κB, JNK and extracellular signalregulated kinase pathway[9]....
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...IL-6 is also involved in the production of autoantibodies and plays a role in arthritic progression through elevated levels of vascular endothelial growth factor[37]....
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...It is considered as a miracle plant having medicinal properties such as anti-inflammatory, anti-diabetic, anti-cancer, anti-oxidant, immunomodulation and wound healing properties[7]....
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