Aloe barbadensis Miller peptide/polypeptide fraction alleviates inflammation through inhibition of proinflammatory cytokines and mediators in vitro and in rats with Freund’s adjuvant-induced hind paw edema
01 Dec 2019-Asian pacific Journal of Tropical Biomedicine (Medknow Publications)-Vol. 9, Iss: 12, pp 524
TL;DR: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
Abstract: Objective: To evaluate the anti-inflammatory potential of peptide/polypeptide fraction of Aloe vera through in vitro and in vivo studies. Methods: The peptide/polypeptide fraction from Aloe vera was obtained through trichloroacetic acid precipitation. The anti-inflammatory property of the peptide/polypeptide fraction was tested by protein denaturation, membrane stabilization assays. The effect of the fraction on RAW 264.7 cell viability was examined by MTT assays. The nitric oxide level was determined through Griess reagent. TNF-α and IL-6 levels were estimated using ELISA kits. In vivo studies were carried out in male Wistar rats through injection of Freund’s adjuvant in the hind paw. Paw edema was measured through the Vernier scale and levels of alanine aminotransferase, aspartate transaminase, TNF-α, IL-6, and secretory phospholipase A2 were estimated through their respective kits after fourteen days of treatment. GraphPad Prism6 was used for analyzing the results. Results: The peptide/polypeptide extract inhibited protein denaturation with an IC50 value of (218.9±15.6) μg/mL and stabilized the membrane of red blood cells with an IC50 value of (275.9±19.1) μg/mL. The extract showed no changes in cell morphology or cytotoxicity up to the concentration of 20 μg/mL in MTT assays. The peptide/polypeptide fraction markedly reduced the levels of proinflammatory markers and mediators in both in vitro and in vivo studies. Conclusions: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
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TL;DR: In this article , immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.
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TL;DR: In this article, immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.
23 citations
TL;DR: This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits, and the potential applications and constraints have been discussed.
Abstract: The therapeutic and pharmacological properties of plant bioactive constituents still continue to be the subject of many researches. Species of the Aloe genus have a history in folklore medicine and they have gained more attention over the years due to their various medicinal properties. Phytochemical studies have revealed that the Aloe species contain a number of constituents, such as polyphenols, phytosterols, polysaccharides, proteins, amino acids, chromones, and mineral elements. A comprehensive evidence-based review on the different constituents of Aloe species is needed in order to understand the benefits imparted by them. This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits. This information will be beneficial for the advancement of new strategies of Aloe formulations with therapeutic and economical value in the near future. Furthermore, the potential applications and constraints have also been discussed so as to provide a wider prospect for research in this field for the benefit of the society.
11 citations
Cites background from "Aloe barbadensis Miller peptide/pol..."
...The flower grows around 90 cm, often being pendulous and consists of yellow tubular corolla around 2–3 cm (Babu et al., 2019)....
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...Apart from that, polyphenols from Aloe species have been reported to possess anti-diabetic potential, anti-inflammatory properties, and wound healing properties (Babu et al., 2019)....
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TL;DR: In this article, the mechanism of action of Aloe vera and its two constituents (Carbohydrates and Polypeptides) in the alleviation of diabetes in streptozotocin-induced diabetic rats through a proteomics approach was investigated.
7 citations
TL;DR: In this article, the role of peptide/polypeptide fraction (PPF) of Aloe vera in the alleviation of diabetes through maintaining the intestinal permeability by regulating the zonulin and GLP-1 levels.
7 citations
References
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Journal Article•
TL;DR: In diabetic rats and diabetic rats fed with A. vera, no change was noticed in the kidney and stomach, while in streptozotocin-induced diabetic rats, the fasting plasma glucose levels were reduced to normal and body weight was found to be increased.
Abstract: An attempt was made to study the beneficial effects of Aloe vera (L.) Burm. fil. in streptozotocin-induced diabetic rats. In diabetic induced rats fed with A. vera (300 mg/kg body wt), the fasting plasma glucose levels were reduced to normal and body weight was found to be increased. In the pancreatic sections of diabetic rats fed with A. vera, the islets were comparable to normal rats. In liver, the changes caused after induction of diabetes are granular cytoplasm, dilated sinusoids, shrunken nuclei and inflammation, which was reduced after feeding with A. vera. Excess proliferation of epithelium in the small intestine was observed in diabetic rats, which was reduced after A. vera feeding. In diabetic rats and diabetic rats fed with A. vera, no change was noticed in the kidney and stomach.
176 citations
"Aloe barbadensis Miller peptide/pol..." refers methods in this paper
...The Aloe vera extract was prepared according to Noor et al[14]....
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...Experimental procedure The animals were randomly allocated to four different groups comprising of six animals each as mentioned below: Group 1: Normal rats (N); Group 2: Freund’s adjuvant-induced rats (IC); Group 3: Freund’s adjuvant-induced rats treated with PPF extract[14] (0....
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TL;DR: The present work contributes to the validation of the anti-inflammatory activity of the plant and may provide some evidence for its folk use and further exploitation.
Abstract: Albuca setosa is widely distributed in the Eastern Cape region of South Africa where its traditional usage is very extensive. This study was aimed to experimentally evaluate the effect of A. setosa water extract (ASWE) on inflammation events such as membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. This study was undertaken using hypotonicity and heat induced erythrocytes haemolysis, heat induced albumin denaturation, carrageenan and dextran induced peritonis and rat paw edema. The results showed that ASWE at a concentration range of 125 - 500 μg/ml significantly (p < 0.01) protects the erythrocyte membrane against lysis induced by heat and hypotonic medium solution. At the dose of 500 and 125 μg/ml, ASWE showed significant (p < 0.01) inhibition of 59 and 65% of protein denaturation of egg albumin. Oral administration of 150 and 300 mg/kg of ASWE significantly (p < 0.05) reduced the total WBC count in rat paw fluid after inflammation induced by carrageenin and in the peritoneal wash after acute inflammation induced by dextran and carrageenan, respectively. The present work contributes to the validation of the anti-inflammatory activity of the plant and may provide some evidence for its folk use and further exploitation.
Key words: Albuca setosa, membrane stabilization, protein denaturation, white blood cell migration, anti-inflammatory activity.
149 citations
"Aloe barbadensis Miller peptide/pol..." refers background in this paper
...The RBC membrane is similar to the lysosomal membrane and hence stabilization of the RBC membrane is one of the assays to determine the anti-inflammatory potential of extracts[33]....
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TL;DR: An updated survey on bioactive peptides present in food crop plants, with a particular focus on immunomodulatory peptides which might be relevant for therapeutic applications is reported, using a bioinformatic approach.
142 citations
"Aloe barbadensis Miller peptide/pol..." refers background in this paper
...Previous studies have reported the anti-inflammatory potential of Aloe vera gel, where the gel exerts its anti-inflammatory property through inhibition of COX2, prostaglandins, bradykinins[8] and through the inhibition of NF-κB, JNK and extracellular signalregulated kinase pathway[9]....
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...Conclusions: The results indicate that the peptide/polypeptide fraction of Aloe vera has antiinflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways....
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...Lunasin derived from soybean exerts its anti-inflammatory activity through inhibition of NF-κB[10] while cyclic peptides from Annona squamosa seeds could reduce proinflammatory cytokines in vitro[11]....
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...The expression of these factors is further activated through nuclear factor (NF-κB) and mitogenactivated protein kinase (MAPK) pathways....
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TL;DR: It is shown for the first time that Aloe vera-mediated strong reduction of IL-1β appears to be the consequence of the reduced expression of both pro-IL-1 β as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways.
134 citations
"Aloe barbadensis Miller peptide/pol..." refers background in this paper
...Previous studies have reported the anti-inflammatory potential of Aloe vera gel, where the gel exerts its anti-inflammatory property through inhibition of COX2, prostaglandins, bradykinins[8] and through the inhibition of NF-κB, JNK and extracellular signalregulated kinase pathway[9]....
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TL;DR: Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels.
Abstract: Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels. TCA precipitation denatures the protein, so it should not be used if the protein must remain in its folded state (e.g., if you want to measure a biochemical activity of the protein).
121 citations