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Journal ArticleDOI

Aloe barbadensis Miller peptide/polypeptide fraction alleviates inflammation through inhibition of proinflammatory cytokines and mediators in vitro and in rats with Freund’s adjuvant-induced hind paw edema

01 Dec 2019-Asian pacific Journal of Tropical Biomedicine (Medknow Publications)-Vol. 9, Iss: 12, pp 524
TL;DR: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
Abstract: Objective: To evaluate the anti-inflammatory potential of peptide/polypeptide fraction of Aloe vera through in vitro and in vivo studies. Methods: The peptide/polypeptide fraction from Aloe vera was obtained through trichloroacetic acid precipitation. The anti-inflammatory property of the peptide/polypeptide fraction was tested by protein denaturation, membrane stabilization assays. The effect of the fraction on RAW 264.7 cell viability was examined by MTT assays. The nitric oxide level was determined through Griess reagent. TNF-α and IL-6 levels were estimated using ELISA kits. In vivo studies were carried out in male Wistar rats through injection of Freund’s adjuvant in the hind paw. Paw edema was measured through the Vernier scale and levels of alanine aminotransferase, aspartate transaminase, TNF-α, IL-6, and secretory phospholipase A2 were estimated through their respective kits after fourteen days of treatment. GraphPad Prism6 was used for analyzing the results. Results: The peptide/polypeptide extract inhibited protein denaturation with an IC50 value of (218.9±15.6) μg/mL and stabilized the membrane of red blood cells with an IC50 value of (275.9±19.1) μg/mL. The extract showed no changes in cell morphology or cytotoxicity up to the concentration of 20 μg/mL in MTT assays. The peptide/polypeptide fraction markedly reduced the levels of proinflammatory markers and mediators in both in vitro and in vivo studies. Conclusions: The results indicate that the peptide/polypeptide fraction of Aloe vera has anti-inflammatory property through inhibition of inflammatory markers and mediators responsible for NF-κB and mitogen-activated protein kinase pathways.
Citations
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Journal ArticleDOI
01 Feb 2022-Peptides
TL;DR: In this article , immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.

23 citations

Journal ArticleDOI
01 Feb 2022-Peptides
TL;DR: In this article, immunomodulatory peptides are used in vaccines as adjuvants which would be extremely desirable, especially in response to pandemics, in order to provide valuable suplements of novel functional food preparation and/or as precursors or active ingredients for drugs design for treatment variety of conditions arising from impaired function of immune system.

23 citations

Journal ArticleDOI
TL;DR: This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits, and the potential applications and constraints have been discussed.
Abstract: The therapeutic and pharmacological properties of plant bioactive constituents still continue to be the subject of many researches. Species of the Aloe genus have a history in folklore medicine and they have gained more attention over the years due to their various medicinal properties. Phytochemical studies have revealed that the Aloe species contain a number of constituents, such as polyphenols, phytosterols, polysaccharides, proteins, amino acids, chromones, and mineral elements. A comprehensive evidence-based review on the different constituents of Aloe species is needed in order to understand the benefits imparted by them. This review presents an overview of the bioactive components of the Aloe genus with emphasis on their anti-diabetic potential and other pharmacological benefits. This information will be beneficial for the advancement of new strategies of Aloe formulations with therapeutic and economical value in the near future. Furthermore, the potential applications and constraints have also been discussed so as to provide a wider prospect for research in this field for the benefit of the society.

11 citations


Cites background from "Aloe barbadensis Miller peptide/pol..."

  • ...The flower grows around 90 cm, often being pendulous and consists of yellow tubular corolla around 2–3 cm (Babu et al., 2019)....

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  • ...Apart from that, polyphenols from Aloe species have been reported to possess anti-diabetic potential, anti-inflammatory properties, and wound healing properties (Babu et al., 2019)....

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Journal ArticleDOI
TL;DR: In this article, the mechanism of action of Aloe vera and its two constituents (Carbohydrates and Polypeptides) in the alleviation of diabetes in streptozotocin-induced diabetic rats through a proteomics approach was investigated.

7 citations

Journal ArticleDOI
TL;DR: In this article, the role of peptide/polypeptide fraction (PPF) of Aloe vera in the alleviation of diabetes through maintaining the intestinal permeability by regulating the zonulin and GLP-1 levels.

7 citations

References
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Journal ArticleDOI
TL;DR: It is demonstrated that aqueous extract of Astragali radix can reduce the suppression of macrophage cell proliferation induced by MTX, and induce IL-1alpha,IL-1beta and IL-6 mRNA expressions in RAW 264.7 macrophages.

74 citations


"Aloe barbadensis Miller peptide/pol..." refers background in this paper

  • ...The high levels of these proinflammatory mediators and cytokines have been implicated in autoimmune disorders[34]....

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Journal ArticleDOI
TL;DR: Multiple sPLA2s are expressed in human RA, in which they may play a role in the augmentation of arachidonate metabolism or exhibit other cell type‐specific functions.
Abstract: Although group IIA secretory phospholipase A2 (sPLA2-IIA) is known to be abundantly present in the joints of patients with rheumatoid arthritis (RA), expression of other sPLA2s in this disease has remained unknown. In this study, we examined the expression and localization of six sPLA2s (groups IIA, IID, IIE, IIF, V and X) in human RA. Immunohistochemistry of RA sections revealed that sPLA2-IIA was generally located in synovial lining and sublining cells and cartilage chondrocytes, sPLA2-IID in lymph follicles and capillary endothelium, sPLA2-IIE in vascular smooth muscle cells, and sPLA2-V in interstitial fibroblasts. Expression levels of these group II subfamily sPLA2s appeared to be higher in severe RA than in inactive RA. sPLA2-X was detected in synovial lining cells and interstitial fibers in both active and inactive RA sections. Expression of sPLA2-IIF was partially positive, yet its correlation with disease states was unclear. Expression of sPLA2 transcripts was also evident in cultured normal human synoviocytes, in which sPLA2-IIA and -V were induced by interleukin-1 and sPLA2-X was expressed constitutively. Adenovirus-mediated expression of sPLA2s in cultured synoviocytes resulted in increased prostaglandin E2 production at low ng x mL(-1) concentrations. Thus, multiple sPLA2s are expressed in human RA, in which they may play a role in the augmentation of arachidonate metabolism or exhibit other cell type-specific functions.

68 citations


"Aloe barbadensis Miller peptide/pol..." refers background in this paper

  • ...The levels of sPLA2 are high in the joints of patients affected with the inflammatory disorder[40,41]....

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Journal ArticleDOI
TL;DR: The results indeed verified the in vitro anti-inflammatory activities of B. racemosa and supported its potential to be used in alleviating gouty arthritis and XO-related diseases.
Abstract: Aim: The present study was conducted to evaluate the in vitro anti-inflammatory activities and total phenolic content (TPC) of methanolic extracts of infloresence axes, endosperms, leaves and pericarps of Barringtonia racemosa L. Methods: The anti-inflammatory study was carried out by assessing the potential through xanthine oxidase (XO) and albumin denaturation inhibition assays. Meanwhile, the total phenolic content (TPC) in the extracts were assessed by Folin Ciocalteu assay. Results: In the XO inhibition assay, the infloresence axes extract was found to exert the highest inhibition capacity at 0.1% (v/v) with 59.54% ± 0.001 inhibition followed by leaves (58.82% ± 0.001), pericarps (57.99% ± 0.003) and endosperms (57.20% ± 0.003) extracts. Similarly in the albumin denaturation inhibition assay, the infloresence axes extract had shown the greatest inhibition capacity with 70.58% ± 0.004 inhibition followed by endosperms (66.80% ± 0.024), leaves (65.29% ± 0.006) and pericarps extracts (43.33% ± 0.002). Meanwhile, for TPC analysis, leaves extract was found to have the highest phenolic content (53.94 ± 0.000 mg GAE/g DW) followed by infloresence axes (31.54 ± 0.001 mg GAE/g DW), endosperms (22.63 ± 0.001 mg GAE/g DW) and the least was found in pericarps (15.54 ± 0.001 mg GAE/g DW). Conclusion: The results indeed verified the in vitro anti-inflammatory activities of B. racemosa and supported its potential to be used in alleviating gouty arthritis and XO-related diseases.

67 citations


"Aloe barbadensis Miller peptide/pol..." refers methods in this paper

  • ...Albumin denaturation inhibition assay Different concentrations of PPF (50, 100, 150, 200 and 250 μg/mL) were mixed with 1% bull serum albumin and phosphate-buffered saline and the assay was carried out according to Osman et al[17] with aspirin as standard drug....

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  • ...Protein denaturation inhibition assay and membrane stabilization assay are used to determine the anti-inflammatory property of the extracts[17,18]....

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Journal ArticleDOI
01 Dec 2000-Blood
TL;DR: Findings suggest that sPLA2 plays a physiologic role in induction of EC migration, and that the effects of s PLA2 on EC migration are mediated, at least in part, by its catalytic activity.

63 citations


"Aloe barbadensis Miller peptide/pol..." refers methods in this paper

  • ...The plasma was used for the estimation of AST and ALT[26], TNF-α, IL-6, and sPLA2[27] levels through respective ELISA kits as per the manufacturer’s protocol....

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Journal ArticleDOI
TL;DR: Research has partially proved that purslane could be considered as a natural anti-inflammatory agent in further applications and significantly reduced LPS-induced synthesis of NO in a dose-dependent manner.

63 citations


"Aloe barbadensis Miller peptide/pol..." refers background in this paper

  • ...MAPK plays a role in the regulation of LPS stimulated NF-κB pathway[42,43]....

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