Journal ArticleDOI
Alterations in neocortical expression of nicotinic acetylcholine receptor mRNAs following unilateral lesions of the rat nucleus basalis magnocellularis.
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TLDR
Results imply regulation of nAChR transcripts by cell to cell interactions and may provide supporting evidence for the occurrence of supersensitivity in deafferentated cholinergic neurons.Abstract:
We investigated the effect of a unilateral lesion of the nucleus basalis magnocellularis (nbm) on the expression of nicotinic acetylcholine receptors (nAChRs) in the rat cerebral cortex. Cortical nAChR concentration as determined by [3H]nicotine binding was unaffected by the nbm lesion. Expression levels of nAChR subunit mRNAs were measured using cDNA clones coding for the receptor subunits, alpha-3, alpha-4, and beta-2. At 1 week postlesion, expression levels of alpha-4, and beta-2 were increased by an average of 82% and 19%, respectively. On the other hand, expression levels of these mRNAs on the lesioned side 4 weeks after lesioning did not differ from those on the control side. Expression of alpha-3 was not altered by the nbm lesion. These results imply regulation of nAChR transcripts by cell to cell interactions. Coincrease of alpha-4 and beta-2 transcripts may provide supporting evidence for the occurrence of supersensitivity in deafferentated cholinergic neurons.read more
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Alteration in nicotine binding sites in Parkinson's disease, Lewy body dementia and Alzheimer's disease: possible index of early neuropathology.
Elaine K. Perry,Christopher Morris,J.A. Court,A. V. T. Cheng,Andrew Fairbairn,Ian G. McKeith,Dorothy Irving,Andy Brown,Robert H. Perry +8 more
TL;DR: Abnormalities of the nicotinic receptor in the diseases examined appear to be closely associated with primary histopathological changes: dopaminergic cell loss in Parkinson's disease and Lewy body dementia, amyloid plaques and tangles in subicular and entorhinal areas in Alzheimer's disease.
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Nicotinic receptor abnormalities in Alzheimer's disease.
Jennifer A. Court,Carmen Martin-Ruiz,Margaret A. Piggott,Dean Spurden,Martin Griffiths,Elaine K. Perry +5 more
TL;DR: Changes in nicotinic acetylcholine receptors in Alzheimer's disease are distinct from those in normal aging and are likely to contribute to clinical features and possibly neuropathology.
Journal ArticleDOI
Cortical cholinergic dysfunction after human head injury
TL;DR: Correlation of choline acetyltransferase activity with synaptophysin immunoreactivity indicated there is a deficit of cholinergic presynaptic terminals in postmortem human brain following head injury.
Journal ArticleDOI
The molecular biology of neuronal nicotinic acetylcholine receptors
TL;DR: The molecular cloning of genes encoding neuronal nicotinic acetylcholine receptors (nAChRs) has made possible a better understanding of the pharmacology and toxicology of cholinergic compounds and may allow for precise control of neurotransmission mediated by acetylCholine in diverse populations of neurons.
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Differential changes in cholinergic markers from selected brain regions after specific immunolesion of the rat cholinergic basal forebrain system
TL;DR: The data support the usefulness of the 192IgG‐saporin conjugate as an appropriate tool to produce cortical cholinergic dysfunction and region‐specific changes in the level of M1‐ and M2‐mAChRs, as well as corresponding receptor gene expression and the lack of effects on cortical nicotinic receptors, may be part of an adaptive mechanism in response to choline degeneration.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Haim Aviv,Philip Leder +1 more
TL;DR: A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
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A rapid radiochemical method for the determination of choline acetyltransferase.
TL;DR: It is shown that the method for isolating labelled ACh by liquid cation exchange using sodium tetraphenylboron (Kalignost) in ethyl butyl ketone can be simplified considerably by using the scintillation mixture as extraction solvent and carrying out the extraction directly in theScintillation vial.