An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei L-lactate dehydrogenase activity induced by a single amino acid substitution.
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...Moreover, the amino acids involved in activation by fructose 1,6-bisphosphate (FDP) (Arg 173 and His 188) were also found in L-nLDH (Figure S1) [28]....
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...This observation can probably be ascribed to the upregulation of, on the one hand, the glycolytic enzymes, including glyceraldehyde phosphate dehydrogenase (GAPDH), triosephosphate isomerase (TPI), and phosphoglycerate kinase (PGK), and on the other hand enzymes of the anaplerotic pathway including phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH) and LDH (Inui et al. 2007)....
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...Indeed, the LDH activity in LPglc267/ pCRB215 (GLK, GAPDH, PFK, TPI, FBA) was higher than that in LPglc279/pCRB215 (GLK, GAPDH, TPI, FBA) both at 2 and 48 h (Fig....
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...To test this possibility, we checked the enzyme activities of LDH and PFK, together with GLK, GAPDH, TPI, and FBA in LPglc267/pCRB215 (GLK, GAPDH, PFK, TPI, FBA) and LPglc279/pCRB215 (GLK, GAPDH, TPI, FBA) at 2 and 48 h in the bioprocess....
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...Together with the LDH activity, the D-lactate yield in LPglc267/pCRB215 (GLK, GAPDH, PFK, TPI, FBA) was also higher than that in LPglc279/pCRB215 (GLK, GAPDH, TPI, FBA) with 90.0±1.4 and 83.8±1.9 %, respectively (Table 2)....
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...In contrast, LPglc267/pCRB215 (GLK, GAPDH, PFK, TPI, FBA) should supply enough fructose-1,6-bisphohate for sufficient LDH activity even after 32 h in the bioprocess because of its increased PFK activity....
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References
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"An absolute requirement of fructose..." refers methods in this paper
...The purity of the enzyme preparations was examined by SDS–PAGE according to Laemmli (Laemmli, 1970)....
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"An absolute requirement of fructose..." refers methods in this paper
...Preparation of the wild-type and mutant L.casei L-LDHs Oligodeoxynucleotide 5 -GAT GTT AGC AGT AGC TTG CCA TAC AGG-3 was purchased from Takara Syuzo and used to replace His205 with Thr. Site-directed mutagenesis was performed with a MUTA-GENE in vitro mutagenesis kit (Bio-RAD) according to Kunkel (Kunkel, 1985)....
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...…the wild-type and mutant L.casei L-LDHs Oligodeoxynucleotide 5 -GAT GTT AGC AGT AGC TTG CCA TAC AGG-3 was purchased from Takara Syuzo and used to replace His205 with Thr. Site-directed mutagenesis was performed with a MUTA-GENE in vitro mutagenesis kit (Bio-RAD) according to Kunkel (Kunkel, 1985)....
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8,157 citations
"An absolute requirement of fructose..." refers background in this paper
...In the case of the B.longum alosteric L-LDH, it has been reported that the allosteric transition can be assumed as two states, the inactive (T) and active (R) states, according to the concerted model demonstrated by Monod et al. (Monod et al., 1965), and the allosteric and catalytic sites communicate with each other through the quaternary structure change (Iwata et al., 1994; Fushinobu et al., 1996, 1998)....
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...…transition can be assumed as two states, the inactive (T) and active (R) states, according to the concerted model demonstrated by Monod et al. (Monod et al., 1965), and the allosteric and catalytic sites communicate with each other through the quaternary structure change (Iwata et al., 1994;…...
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