Journal ArticleDOI
An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow.
Manfred B. Lutz,Nicole A. Kukutsch,A. L. J. Ogilvie,Susanne Rössner,Franz Koch,Nikolaus Romani,Gerold Schuler +6 more
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TLDR
This method allows by simple means the generation of high numbers of murine DC with very low B cell or granulocyte contaminations, which will be valuable to study DC biology notably at the molecular level.About:
This article is published in Journal of Immunological Methods.The article was published on 1999-02-01. It has received 2935 citations till now. The article focuses on the topics: Bone marrow & Dendritic cell.read more
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Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy.
Lionel Apetoh,François Ghiringhelli,Antoine Tesniere,Antoine Tesniere,Antoine Tesniere,Michel Obeid,Michel Obeid,Michel Obeid,Carla Ortiz,Carla Ortiz,Carla Ortiz,Alfredo Criollo,Alfredo Criollo,Alfredo Criollo,Grégoire Mignot,Grégoire Mignot,Grégoire Mignot,M. Chiara Maiuri,Evelyn Ullrich,Evelyn Ullrich,Evelyn Ullrich,Patrick Saulnier,Huan Yang,Sebastian Amigorena,Bernard Ryffel,Franck J. Barrat,Paul Saftig,Francis Lévi,Francis Lévi,Rosette Lidereau,Catherine Noguès,Jean-Paul Mira,Agnès Chompret,Virginie Joulin,Françoise Clavel-Chapelon,Jean Bourhis,Fabrice Andre,Suzette Delaloge,T. Tursz,Guido Kroemer,Guido Kroemer,Guido Kroemer,Laurence Zitvogel +42 more
TL;DR: A previously unrecognized pathway for the activation of tumor antigen–specific T-cell immunity that involves secretion of the high-mobility-group box 1 (HMGB1) alarmin protein by dying tumor cells and the action of HMGB1 on Toll-like receptor 4 (TLR4) expressed by dendritic cells (DCs) is described.
Journal ArticleDOI
CD4 + CD25 + regulatory T cells control Leishmania major persistence and immunity
Yasmine Belkaid,Yasmine Belkaid,Ciriaco A. Piccirillo,Susana Mendez,Ethan M. Shevach,David L. Sacks +5 more
TL;DR: It is shown that the persistence of Leishmania major in the skin after healing in resistant C57BL/6 mice is controlled by an endogenous population of CD4+CD25+ regulatory T cells, indicating that the equilibrium established between effector and regulatory T Cells in sites of chronic infection might reflect both parasite and host survival strategies.
Journal ArticleDOI
Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-κB pathway
TL;DR: It is reported here that heat shock proteins (HSP), the most abundant and conserved mammalian molecules, constitute such an internal signal that provides a unified mechanism for response to internal and external stimuli.
Journal ArticleDOI
Rapid leukocyte migration by integrin-independent flowing and squeezing
Tim Lämmermann,Bernhard L. Bader,Susan J. Monkley,Tim Worbs,Roland Wedlich-Söldner,Karin Hirsch,Markus Keller,Reinhold Förster,David R. Critchley,Reinhard Fässler,Michael Sixt +10 more
TL;DR: It is shown here that functional integrins do not contribute to migration in three-dimensional environments, and these cells migrate by the sole force of actin-network expansion, which promotes protrusive flowing of the leading edge.
Journal ArticleDOI
Induced recruitment of NK cells to lymph nodes provides IFN-gamma for T(H)1 priming.
Alfonso Martín-Fontecha,Lindy L. Thomsen,Sara Brett,Craig Gerard,Martin Lipp,Antonio Lanzavecchia,Federica Sallusto +6 more
TL;DR: It is shown in mice that natural killer (NK) cells are rapidly recruited in a CCR7-independent, CXCR3-dependent manner to lymph nodes on stimulation by the injection of mature DCs, and an induced pathway of NK cell migration in antigen-stimulated lymph nodes is identified.
References
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Current protocols in immunology
TL;DR: Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, and animal models of autoimmune and inflammatory diseases.
Journal ArticleDOI
Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.
TL;DR: Cultured DCs are as efficient as antigen-specific B cells in presenting tetanus toxoid (TT) to specific T cell clones and their efficiency of antigen presentation can be further enhanced by specific antibodies via FcR- mediated antigen uptake.
Journal ArticleDOI
The dendritic cell system and its role in immunogenicity
TL;DR: Dendritic cells are specialized to mediate several physiologic components of immunogenicity such as the acquisition of antigens in tissues, the migration to lymphoid organs, and the identification and activation of antigen-specific T cells.
Journal ArticleDOI
Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor.
K Inaba,M Inaba,Nikolaus Romani,H Aya,Masashi Deguchi,Susumu Ikehara,Shigeru Muramatsu,Ralph M. Steinman +7 more
TL;DR: The methodology for inducing dendritic cell growth that was recently described for mouse blood now has been modified to MHC class II- negative precursors in marrow, and this feature should prove useful for future molecular and clinical studies of this otherwise trace cell type.
Journal ArticleDOI
Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products.
TL;DR: The capacity of DCs to capture and process antigen could be modulated by exogenous stimuli was investigated and it was found that DCs respond to tumor necrosis factor alpha, CD40 ligand, IL-1, and lipopolysaccharide with a coordinate series of changes that include downregulation of macropinocytosis and Fc receptors, disappearance of the class II compartment, and upregulation of adhesion and costimulatory molecules.